Identification, Association of Natural Variation and Expression Analysis of ZmNAC9 Gene Response to Low Phosphorus in Maize Seedling Stage.
Identification, Association of Natural Variation and Expression Analysis of ZmNAC9 Gene Response to Low Phosphorus in Maize Seedling Stage.
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DOI：
10.3390/plants9111447
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发表时间：
2020-10-27
期刊：
影响因子：
--
通讯作者：
Gao S
中科院分区：
文献类型：
--
作者：
Sahito JH;Luo B;Khalid MHB;Sahito HA;Ali A;Ghulam Nabi Gishkori Z;Zhang X;He X;Zhong H;Zhen C;Ma P;Liu D;Wu L;Gao S
磷是维持玉米生产力的必需常量元素，低磷胁迫是玉米生长和产量的限制因子。通过分子育种提高玉米植株对低磷的耐性是提高作物产量的有效途径。本研究以111个玉米自交系为材料，对ZmNAC 9基因的有利等位基因和核苷酸多样性进行了鉴定，该基因在低磷胁迫下的根构型调控中发挥着重要作用。在低磷和充足磷条件下，22个幼苗性状均存在显著差异，在111个自交系中，ZmNAC 9共检测到41个多态性位点，其中包括32个单核苷酸多态性（SNP）和9个插入和缺失（InDel）。在41个多态性研究位点中，共有39个多态位点与20个性状（除枝干重和叉干重）相关，其中6个位点与不同数量的耐低磷根性状指数值高度显著相关，采用混合线性模型（MLM）在−log10 P = 3.61。此外，29个多态性位点在磷充足和32个多态性位点在磷缺乏条件下显着相关的幼苗性状的多样性，其中5个多态性位点（位置S327，S513，S514，S520，和S827）在低磷和正常磷条件下与多个幼苗性状密切相关。在极显著位点中，大多数位点在低磷、正常磷和低磷性状指数值下与根系性状相关。111个自交系在r2 > 1.0时连锁不平衡（LD）较强。在111个株系中，5个显著位点对单倍型的影响得到验证，其中有利等位基因S520对低磷条件下的根干重表现出正效应。此外，表达模式证实ZmNAC9在耐磷自交系178的根中受到低P的高度诱导。此外，ZmNAC 9蛋白编码的亚细胞定位位于细胞质和细胞核中。携带更多有利等位基因的单倍型对表型变异具有上级效应，有助于玉米分子育种中分子标记的开发。总之，这项研究的发现可能会导致ZmNAC9和可能参与玉米低磷胁迫响应的基因的进一步功能。
Phosphorus (P) is an essential macroelement supporting maize productivity and low-P stress is a limiting factor of maize growth and yield. Improving maize plant tolerance to low P through molecular breeding is an effective alternative to increase crop productivity. In this study, a total of 111 diverse maize inbred lines were used to identify the favorable alleles and nucleotide diversity of candidate ZmNAC9, which plays an important role in response to low P and regulation in root architecture. A significant difference was found under low- and sufficient-P conditions for each of the 22 seedling traits, and a total of 41 polymorphic sites including 32 single nucleotide polymorphisms (SNPs) and 9 insertion and deletions (InDels) were detected in ZmNAC9 among 111 inbred lines. Among the 41 polymorphic studied sites, a total of 39 polymorphic sites were associated with 20 traits except for the dry weight of shoots and forks, of which six sites were highly significantly associated with a diverse number of low-P tolerant root trait index values by using a mixed linear model (MLM) at −log10 P = 3.61. In addition, 29 polymorphic sites under P-sufficient and 32 polymorphic sites under P-deficient conditions were significantly associated with a diverse number of seedling traits, of which five polymorphic sites (position S327, S513, S514, S520, and S827) were strongly significantly associated with multiple seedling traits under low-P and normal-P conditions. Among highly significant sites, most of the sites were associated with root traits under low-P, normal-P, and low-P trait index values. Linkage disequilibrium (LD) was strong at (r2 > 1.0) in 111 inbred lines. Furthermore, the effect of five significant sites was verified for haplotypes in 111 lines and the favorable allele S520 showed a positive effect on the dry weight of roots under the low-P condition. Furthermore, the expression pattern confirmed that ZmNAC9 was highly induced by low P in the roots of the P-tolerant 178 inbred line. Moreover, the subcellular localization of ZmNAC9 encoded by protein was located in the cytoplasm and nucleus. Haplotypes carrying more favorable alleles exhibited superior effects on phenotypic variation and could be helpful in developing molecular markers in maize molecular breeding programs. Taken together, the finding of this study might lead to further functions of ZmNAC9 and genes that might be involved in responses to low-P stress in maize.
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影响因子：
11.6
作者：
Hamburger, D;Rezzonico, E;Poirier, Y
通讯作者：
Poirier, Y
影响因子：
2.9
作者：
Ching A;Caldwell KS;Jung M;Dolan M;Smith OS;Tingey S;Morgante M;Rafalski AJ
通讯作者：
Rafalski AJ
影响因子：
5.8
作者：
Bluemmel, Michael;Grings, Elaine;Erenstein, Olaf
通讯作者：
Erenstein, Olaf
影响因子：
5.8
作者：
Bradbury, Peter J.;Zhang, Zhiwu;Buckler, Edward S.
通讯作者：
Buckler, Edward S.
影响因子：
3.1
作者：
Chai, Yuchao;Hao, Xiaomin;Li, Jiansheng
通讯作者：
Li, Jiansheng