Genetic diversity analysis using simple sequence repeat markers in soybean

Genetic diversity analysis using simple sequence repeat markers in soybean
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使用简单序列重复标记进行大豆遗传多样性分析

DOI:
10.1017/s1479262114000331
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发表时间:
2014-07
期刊:
Plant Genetic Resources: Characterization and Utilization
影响因子:
--
通讯作者:
Yu, Deyue
Yu, Deyue
中科院分区:
其他
文献类型:
--
作者:
Zhang, Guozheng;Zhang, Dan;Hao, Derong;Yu, Deyue

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为了评价野生大豆和栽培大豆的遗传多样性,并确定它们之间的遗传关系,利用74个SSR标记对127份野生大豆和219份栽培大豆进行了基因分型。研究结果表明,野生大豆的GD大于栽培大豆。共检测到924个等位基因,平均每个位点12.49个。在219份栽培大豆材料中检测到687个等位基因,平均每个位点9.28个等位基因;在127份野生大豆材料中检测到835个等位基因,平均每个位点11.28个等位基因。在346份大豆材料中共检测到237个野生大豆特异等位基因和89个栽培大豆特异等位基因,占总等位基因数的35.28%。主坐标分析和基于Nei遗传距离的系统发育分析结果表明,供试材料可分为野生大豆和栽培大豆两大类。这些结果将增加我们对野生大豆和栽培大豆之间的遗传差异和关系的理解,并为制定未来的育种策略以提高大豆产量提供信息。
To evaluate the genetic diversity (GD) of wild and cultivated soybeans and determine the genetic relationships between them, in this study, 127 wild soybean accessions and 219 cultivated soybean accessions were genotyped using 74 simple sequence repeat (SSR) markers. The results of the study revealed that the GD of the wild soybeans exceeded that of the cultivated soybeans. In all, 924 alleles were detected in the 346 soybean accessions using 74 SSRs, with an average of 12.49 alleles per locus. In the 219 cultivated soybean accessions, 687 alleles were detected, with an average of 9.28 alleles per locus; in the 127 wild soybean accessions, 835 alleles were detected, with an average of 11.28 alleles per locus. We identified 237 wild-soybean-specific alleles and 89 cultivated-soybean-specific alleles in the 346 soybean accessions, and these alleles accounted for 35.28% of all the alleles in the sample. Principal coordinates analysis and phylogenetic analysis based on Nei's genetic distance indicated that all the accessions could be classified into two major clusters, corresponding to wild and cultivated soybeans. These results will increase our understanding of the genetic differences and relationships between wild and cultivated soybeans and provide information to develop future breeding strategies to improve soybean yield.
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