Association mapping of yield-related traits and SSR markers in wild soybean (Glycine soja Sieb. and Zucc.).

Association mapping of yield-related traits and SSR markers in wild soybean (Glycine soja Sieb. and Zucc.).
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DOI:
10.1270/jsbbs.63.441
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发表时间:
2014-03
期刊:
影响因子:
2.4
通讯作者:
Yu D
Yu D
中科院分区:
农林科学3区
文献类型:
--
作者:
Hu Z;Zhang D;Zhang G;Kan G;Hong D;Yu D

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野生大豆是栽培大豆的祖先,是大豆育种的重要基因库。为从野生大豆中筛选出增产数量性状位点(QTL)或基因,对113份野生大豆材料的5个产量相关性状进行了表型分析,并利用85个SSR标记进行了基因型分析。85个SSR标记共检测到892个等位基因,平均10.49个,对应的PIC值在0.07 ~ 0.92之间,平均0.73。每个SSR标记的遗传多样性在0.07 ~ 0.93之间,平均为0.75。共鉴定出18对SSR标记。两个SSR标记,sct_010和satt 316,这是与单株产量在两个实验地点稳定表达了两年。研究结果表明,关联作图是一种有效的野生大豆QTL定位方法。
Wild soybean, the progenitor of cultivated soybean, is an important gene pool for ongoing soybean breeding efforts. To identify yield-enhancing quantitative trait locus (QTL) or gene from wild soybean, 113 wild soybeans accessions were phenotyped for five yield-related traits and genotyped with 85 simple sequence repeat (SSR) markers to conduct association mapping. A total of 892 alleles were detected for the 85 SSR markers, with an average 10.49 alleles; the corresponding PIC values ranged from 0.07 to 0.92, with an average 0.73. The genetic diversity of each SSR marker ranged from 0.07 to 0.93, with an average 0.75. A total of 18 SSR markers were identified for the five traits. Two SSR markers, sct_010 and satt316, which are associated with the yield per plant were stably expressed over two years at two experimental locations. Our results suggested that association mapping can be an effective approach for identifying QTL from wild soybean.
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