The effect of lung alpha-tocopherol content on the acute toxicity of nitrogen dioxide.
The effect of lung alpha-tocopherol content on the acute toxicity of nitrogen dioxide.
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肺α-生育酚含量对二氧化氮急性毒性的影响。
DOI：
10.1016/0041-008x(86)90138-9
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发表时间：
1986
影响因子：
3.8
通讯作者：
Mavis,RD
中科院分区：
文献类型：
--
作者：
Guth,DJ;Mavis,RD
将肺维生素E含量不同的三组大鼠分别暴露于0、10、20、30和40ppm的NO2中4h，研究肺维生素E含量对NO2早期直接肺损伤的影响。用含d-μ-生育酚醋酸酯0、10和1000 mg/kg的半纯饲料饲养动物，肺维生素E含量分别为3.24、17.4和87.7mgg/肺。染毒4小时后立即处死动物，通过测定肺灌洗液中蛋白质、唾液酸、乳酸脱氢酶(LDH)、苹果酸脱氢酶(MDH)、葡萄糖-6-磷酸脱氢酶(GDH)、酸性磷酸酶(AP)和芳基硫酸酯酶(AS)的含量来评估肺损伤。蛋白质、唾液酸、AP和As的增加不受不同维生素E含量的影响，而与0和10 mg/kg饲料组相比，1000 mg/kg饲料组LDH、MDH和GDH的增加显著减弱。除了游离细胞中硫代巴比妥酸反应物质略有增加外，通过共轭双烯测量或硫代巴比妥酸反应物质检测不到暴露于NO2的肺中的脂质过氧化。这些结果提示了NO2对肺的两种损伤机制。一些灌洗参数的出现被高维生素E减弱，这与脂质过氧化是导致其出现的损害的必要事件是一致的，尽管整个肺的脂质过氧化指标缺乏变化，表明过氧化只发生在非常有限的程度。不受肺维生素E含量影响的灌洗参数明显出现在呼吸道中，这是不涉及脂质过氧化的事件的结果。
The effect of lung vitamin E content on early direct damage to lung by NO2was studied by exposing three groups of rats differing in lung vitamin E content to 0, 10, 20, 30, and 40 ppm NO2for 4 hr. Lung vitamin E contents of 3.24, 17.4, and 87.7 μg/lung were obtained by maintaining animals on semipurified diets containing 0, 10, or 1000 mg/kg of d-α-tocopherol acetate. Animals were sacrificed immediately after the 4-hr exposure and lung damage was assessed by assaying the lung lavage content of protein, sialic acid, lactate dehydrogenase (LDH), malate dehydrogenase (MDH), glucose-6-phosphate dehydrogenase (GDH), acid phosphatase (AP), and aryl sulfatase (AS), all of which increase in lavage fluid in a concentration-dependent manner over the range of NO2concentrations used. Increases in lavagable protein, sialic acid, AP, and AS were not affected by the different vitamin E contents, while the increases in LDH, MDH, and GDH were significantly attenuated in the 1000-mg/kg diet group relative to the 0- and 10-mg/kg diet groups. Lipid peroxidation was not detectable in NO2-exposed lungs by either conjugated diene measurement or thiobarbituric-acid-reactive materials, with the exception of a slight increase in thiobarbituric-acid-reactive material in free cells. These results suggest two mechanisms of NO2damage to lung. The attenuation of the appearance of some lavage parameters by high vitamin E is consistent with lipid peroxidation as a necessary event in the damage responsible for their appearance, although the lack of change in indicators of lipid peroxidation in the whole lung suggests that peroxidation occurs to only a very limited extent. The lavage parameters which are unaffected by lung vitamin E content apparently appear in airways as a result of events not involving lipid peroxidation.
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血浆普萘洛尔水平在人体 β-肾上腺素能阻断定量评估中的应用
影响因子：
--
作者：
D. Coltart;D. Shand
通讯作者：
D. Shand
高效液相色谱法测定血浆中普萘洛尔和4-羟基普萘洛尔。
DOI：
--
发表时间：
1981
期刊：
Journal of Pharmacy and Science
影响因子：
--
作者：
M. Rosseel;M. Bogaert
通讯作者：
M. Bogaert
影响因子：
7.3
作者：
FITZ GERALD, JD;ODONNELL, SR
通讯作者：
ODONNELL, SR
通过高压液相色谱法测量人的普萘洛尔和 4-羟基普萘洛尔的血浆浓度。
DOI：
--
发表时间：
1979
期刊：
Life Science
影响因子：
--
作者：
A. Taburet;Addison A. Taylor;Jerry R. Mitchell;Douglas E. Rollins;James L. Pool
通讯作者：
James L. Pool
一种改进的、灵敏的液相色谱方法，用于通过荧光检测测量普萘洛尔。
影响因子：
3.8
作者：
Koshakji,RP;Wood,AJ
通讯作者：
Wood,AJ