Transcriptome Profiling Provides New Insights into the Molecular Mechanism Underlying the Sensitivity of Cotton Varieties to Mepiquat Chloride.
Transcriptome Profiling Provides New Insights into the Molecular Mechanism Underlying the Sensitivity of Cotton Varieties to Mepiquat Chloride.
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DOI：
10.3390/ijms23095043
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发表时间：
2022-05-02
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
甲吡喹氯(MC)是一种广泛应用于棉花生产的植物生长调节剂，用于控制棉花植株的营养过度生长，以达到高产所需的理想植株结构。棉花品种对多氯联苯的反应不同，但对品种差异的分子机制知之甚少。本研究以对MC敏感性不同的两个陆地棉品种(XLZ74和SD1068)为材料，进行了比较转录组分析，旨在了解造成品种间MC敏感性差异的分子机制。这两个品种分别在喷药后1、3和6天(DPS)用MC或水处理3个时间点的RNA-SEQ数据。通过基因本体论(GO)和京都基因与基因组百科全书(KEGG)分析，比较了XLZ74(6252)和SD1068(6163)MC和模拟处理间差异表达基因的GO术语和KEGG途径。在两个品种中，MC似乎对植物激素的信号转导、赤霉素(GAs)和油菜素类固醇(BRs)的生物合成以及转录因子(TF)的谱产生了不同的影响。转录结果进一步与幼茎中植物激素含量的变化相一致。MC对两个品种的GA分解代谢基因GA2ox均有较高的诱导作用，特别是在SD1068中，与SD1068中GA4的表达显著降低相一致。MC对两个品种的AUX/IAA、SAUR和CKX基因均有诱导作用，但对SD1068的影响更大，喷施后6d，IAA含量显著降低，CTK含量显著升高。与BR生物合成相关的基因在SD1068中表达下调，而在XLZ74中没有表达。此外，MC处理的SD1068比MC处理的XLZ74有更多的转录因子下调，并且两个品种的淀粉和蔗糖代谢相关基因谱有很大的不同，MC处理分别下调和上调了SD1068和XLZ74的转录因子。综上所述，这些结果表明，尽管MC处理对不同MC敏感性的棉花品种具有相同或相似的生物学途径，但影响的程度是不同的，导致它们的表型结果不同。MC在与生长迟缓相关的生物过程中的定量效应如何被调控仍然是一个悬而未决的问题。
Mepiquat chloride (MC) is a plant growth regulator widely used in cotton production to control vegetative overgrowth of cotton plants to achieve ideal plant architecture required for high yielding. Cotton varieties respond differently to MC application, but there is little information about the molecular mechanisms underlying the varietal difference. In this study, comparative transcriptome analysis was conducted by using two Upland cotton varieties with different sensitivity (XLZ74, insensitive; SD1068, sensitive) to MC treatment, aiming to understand the molecular mechanisms responsible for varietal difference of MC sensitivity. RNA-seq data were generated from the two varieties treated with MC or water at three time points, 1, 3 and 6 days post-spray (dps). Genes differentially expressed between the MC and mock treatments of XLZ74 (6252) and SD1068 (6163) were subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses to compare the enriched GO terms and KEGG pathways between the two varieties. Signal transduction of phytohormones, biosynthesis of gibberellins (GAs) and brassinosteroids (BRs) and profiles of transcription factors (TFs) seemed to be differentially affected by MC in the two varieties. The transcriptomic results were further consolidated with the content changes of phytohormones in young stem. Several GA catabolic genes, GA2ox, were highly induced by MC in both varieties especially in SD1068, consistent with a more significant decrease in GA4 in SD1068. Several AUX/IAA and SAUR genes and CKX genes were induced by MC in both varieties, but with a more profound effect observed in SD1068 that showed a significant reduction in indole-3-acetic acid (IAA) and a significant increase in cytokinin (CTK) at 6 days post-spray (dps). BR biosynthesis-related genes were downregulated in SD1068, but not in XLZ74. Additionally, more downregulated TFs were observed in MC-treated SD1068 than in MC-treated XLZ74, and the two varieties had very different profiles of genes involved in starch and sucrose metabolism, with those of SD1068 and XLZ74 being downregulated and upregulated by MC treatment, respectively. Together, these results indicate that although the same or similar biological pathways are affected by MC treatment in cotton varieties showing different MC sensitivity, the extent of effect is variable, leading to their different phenotypic outcomes. How the quantitative effect of MC on the biological processes associated with growth retardation is regulated is still an open question.
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影响因子：
3.7
作者：
Cheon J;Fujioka S;Dilkes BP;Choe S
通讯作者：
Choe S
影响因子：
6.5
作者：
Kloosterman, B.;Visser, R. G. F.;Bachem, C. W. B.
通讯作者：
Bachem, C. W. B.
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
4.4
作者：
Chen R;Fan Y;Zhou H;Mo S;Zhou Z;Yan H;Luo T;Huang X;Weng M;Lakshmanan P;Li Y;Qiu L;Wu J
通讯作者：
Wu J
影响因子：
30.8
作者：
Hu, Yan;Chen, Jiedan;Zhang, Tianzhen
通讯作者：
Zhang, Tianzhen