Identification of excretory and secretory proteins from Haemonchus contortus inducing a Th9 immune response in goats.
Identification of excretory and secretory proteins from Haemonchus contortus inducing a Th9 immune response in goats.
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鉴定山羊中诱导 Th9 免疫反应的捻转血矛线虫的排泄和分泌蛋白
DOI：
10.1186/s13567-022-01055-8
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发表时间：
2022-05-21
影响因子：
4.4
通讯作者：
中科院分区：
文献类型：
--
作者：
Th 9细胞已被证明在抗寄生虫免疫、病原微生物感染和变态反应中起关键作用。已有研究表明，捻转血矛线虫分泌和排泄蛋白（HcESPs）可诱导Th 9细胞增殖，并改变Th 9细胞免疫应答中IL-9的转录水平及其相关通路。然而，HcESP中诱导Th 9免疫应答的确切分子尚不清楚。本研究采用流式细胞术、免疫共沉淀（Co-IP）和鸟枪液相色谱串联质谱（LC-MS/MS）技术，从HcESP中鉴定出218个可能与山羊Th 9细胞相互作用的蛋白。通过与HcESP体外培养的Th 9细胞，鉴定了40个结合蛋白。在体内，分别在感染后7、15、35和50天（dpi）鉴定出38、47、42和142个结合蛋白。另外，在218个HcESP中，有2个HcESP（DNA/RNA helicase domain containing protein，HcDR）和GATA转录因子（GATA transcription factor，HcGATA）与山羊外周血单个核细胞（peripheral blood mononuclear cells，PBMC）共孵育后，可诱导Th 9细胞增殖并促进IL-9的表达。本研究代表了蛋白质组学指导的Th 9细胞和HcESP之间的相互作用的调查。这为在HcESP中寻找免疫刺激性抗原提供了新的途径，并为免疫介导的H.扭体病
Th9 cells have been shown to play crucial roles in anti-parasite immunity, pathogenic microbe infection, and allergy. Previous studies have demonstrated thatHaemonchus contortusexcretory and secretory proteins (HcESPs) induce the proliferation of Th9 cells and alter the transcriptional level of IL-9 as well as its related pathways in the Th9 immune response after infection. However, the exact molecule(s) in HcESPs inducing the Th9 immune response is not yet known. In this study, flow cytometry, co-immunoprecipitation (Co-IP) and shotgun liquid chromatography tandem-mass spectrometry (LC–MS/MS) were used, and a total of 218 proteins from HcESPs that might interact with goat Th9 cells were identified. By in vitro culture of Th9 cells with HcESPs, 40 binding proteins were identified. In vivo, 38, 47, 42 and 142 binding proteins were identified at 7, 15, 35 and 50 days post-infection (dpi), respectively. Furthermore, 2 of the 218 HcESPs, named DNA/RNA helicase domain containing protein (HcDR) and GATA transcription factor (HcGATA), were confirmed to induce the proliferation of Th9 cells and promote the expression of IL-9 when incubated with goat peripheral blood mononuclear cells (PBMCs). This study represents a proteomics-guided investigation of the interactions between Th9 cells and HcESPs. It provides a new way to explore immunostimulatory antigens among HcESPs and identifies candidates for immune-mediated prevention ofH. contortusinfection.
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影响因子：
3.7
作者：
Gadahi JA;Wang S;Bo G;Ehsan M;Yan R;Song X;Xu L;Li X
通讯作者：
Li X
影响因子：
7.3
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Chen, Mark J.;Dixon, Jack E.;Manning, Gerard
通讯作者：
Manning, Gerard
影响因子：
2.2
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BEHNKE, JM;WAHID, FN;GOYAL, PK
通讯作者：
GOYAL, PK
影响因子：
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作者：
Fukushige, T;Hawkins, MG;McGhee, JD
通讯作者：
McGhee, JD
影响因子：
5.4
作者：
Faulkner, H;Humphreys, N;Grencis, R
通讯作者：
Grencis, R