Developmentally regulated cell cycle dependence of swelling-activated anion channel activity in the mouse embryo.
Developmentally regulated cell cycle dependence of swelling-activated anion channel activity in the mouse embryo.
复制标题
小鼠胚胎中肿胀激活阴离子通道活性的发育调节细胞周期依赖性。
作者：
M. Kolajova;M. Hammer;J. L. Collins;J. Baltz
由细胞体积增加激活的阴离子通道是几乎普遍存在的细胞体积调节机制，包括在早期植入前小鼠胚胎中。在这里，我们表明，肿胀激活的阴离子电流（I（Cl，膨胀））在早期小鼠胚胎是细胞周期依赖性的，而且这种依赖性是发育调节。I（Cl，swell）存在于小鼠卵母细胞第一次减数分裂前期（萌发囊泡期）和第二次减数分裂中期未受精的成熟卵母细胞中，并在受精后的1-细胞和2-细胞期持续存在。发现I（Cl，膨胀）在1细胞期结束时的中期保持不变。然而，I（Cl，膨胀）在前期下降，并成为几乎检测不到进入中期后，在2-细胞阶段结束。进入前期/中期是2-细胞期结束时I（Cl，膨胀）降低所必需的，因为它在G（2）晚期停滞的2-细胞胚胎中无限期持续。有相当多的证据表明，I（Cl，膨胀）的通道不仅对无机阴离子是可渗透的，而且对有机渗透剂也是如此。我们发现了一个类似的模式的细胞周期和发育依赖性的1-细胞和2-细胞阶段的肿胀诱导的渗透性增加的有机渗透剂甘氨酸。因此，进入中期使I（Cl，膨胀）在胚胎中失活，但仅在发育进展通过2-细胞阶段之后。
Anion channels activated by increased cell volume are a nearly ubiquitous mechanism of cell volume regulation, including in early preimplantation mouse embryos. Here, we show that the swelling-activated anion current (I(Cl,swell)) in early mouse embryos is cell-cycle dependent, and also that this dependence is developmentally regulated. I(Cl,swell) is present both in first meiotic prophase (germinal vesicle stage) mouse oocytes and in unfertilized mature oocytes in second meiotic metaphase, and it persists after fertilization though the 1-cell and 2-cell stages. I(Cl,swell) was found to remain unchanged during metaphase at the end of the 1-cell stage. However, I(Cl,swell) decreased during prophase and became nearly undetectable upon entry into metaphase at the end of the 2-cell stage. Entry into prophase/metaphase was required for the decrease in I(Cl,swell) at the end of the 2-cell stage, since it persisted indefinitely in 2-cell embryos arrested in late G(2). There is considerable evidence that the channel underlying I(Cl,swell) is not only permeable to inorganic anions, but to organic osmolytes as well. We found a similar pattern of cell cycle and developmental dependence in the 1-cell and 2-cell stages for the swelling-induced increase in permeability to the organic osmolyte glycine. Thus, entry into metaphase deactivates I(Cl,swell) in embryos, but only after developmental progression through the 2-cell stage.
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影响因子：
--
作者：
J. Lawitts;J. Biggers
通讯作者：
J. Lawitts;J. Biggers
向外整流、膨胀激活的阴离子通道的分子特性：重新评估 pICln 的时间。
DOI：
10.1085/jgp.111.5.617
发表时间：
1998
期刊：
The Journal of general physiology
影响因子：
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作者：
Strange,K
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Strange,K
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DOI：
10.1016/0005-2736(94)90175-9
发表时间：
1994
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
VanWinkle,LJ;Patel,M;Wasserlauf,HG;Dickinson,HR;Campione,AL
通讯作者：
Campione,AL
影响因子：
56.9
作者：
BLOCK, ML;MOODY, WJ
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MOODY, WJ
小鼠卵母细胞减数分裂停滞恢复过程中核和细胞质重组的形态动力学。
DOI：
--
发表时间：
1989
期刊：
Progress in clinical and biological research
影响因子：
--
作者：
VanBlerkom,J
通讯作者：
VanBlerkom,J