A Nestin-cre transgenic mouse is insufficient for recombination in early embryonic neural progenitors.
A Nestin-cre transgenic mouse is insufficient for recombination in early embryonic neural progenitors.
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DOI：
10.1242/bio.20122287
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发表时间：
2012-12-15
期刊：
影响因子：
2.4
通讯作者：
Ghashghaei HT
中科院分区：
文献类型：
--
作者：
Liang H;Hippenmeyer S;Ghashghaei HT
Nestin-cre transgenic mice have been widely used to direct recombination to neural stem cells (NSCs) and intermediate neural progenitor cells (NPCs). Here we report that a readily utilized, and the only commercially available, Nestin-cre line is insufficient for directing recombination in early embryonic NSCs and NPCs. Analysis of recombination efficiency in multiple cre-dependent reporters and a genetic mosaic line revealed consistent temporal and spatial patterns of recombination in NSCs and NPCs. For comparison we utilized a knock-in Emx1cre line and found robust recombination in NSCs and NPCs in ventricular and subventricular zones of the cerebral cortices as early as embryonic day 12.5. In addition we found that the rate of Nestin-cre driven recombination only reaches sufficiently high levels in NSCs and NPCs during late embryonic and early postnatal periods. These findings are important when commercially available cre lines are considered for directing recombination to embryonic NSCs and NPCs.
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影响因子：
25
作者：
Madisen L;Zwingman TA;Sunkin SM;Oh SW;Zariwala HA;Gu H;Ng LL;Palmiter RD;Hawrylycz MJ;Jones AR;Lein ES;Zeng H
通讯作者：
Zeng H
影响因子：
16.2
作者：
Hippenmeyer, Simon;Youn, Yong Ha;Luo, Liqun
通讯作者：
Luo, Liqun
影响因子：
3.4
作者：
Isaka, F;Ishibashi, M;Kageyama, R
通讯作者：
Kageyama, R
影响因子：
1.5
作者：
Dubois, Nicole C.;Hofmann, Denise;Trumpp, Andreas
通讯作者：
Trumpp, Andreas
影响因子：
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作者：
Haruyama, Naoto;Cho, Andrew;Kulkarni, Ashok B
通讯作者：
Kulkarni, Ashok B