Characterization of mammalian selenoprotein o: a redox-active mitochondrial protein.
Characterization of mammalian selenoprotein o: a redox-active mitochondrial protein.
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DOI：
10.1371/journal.pone.0095518
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Lee SR
中科院分区：
文献类型：
--
作者：
Han SJ;Lee BC;Yim SH;Gladyshev VN;Lee SR
硒蛋白具有多种生物学功能，其中大部分与氧化还原调控有关。然而，大约一半的哺乳动物硒蛋白的功能是未知的。其中一种蛋白质是硒蛋白O（SelO），它是哺乳动物中最大的硒蛋白，在包括细菌和酵母在内的多种生物体中发现了同源物。在这里，我们报告哺乳动物SelO的表征。这种蛋白质的表达可以验证在HEK 293 T细胞的细胞与75硒的代谢标记，并取消时，硒代半胱氨酸取代丝氨酸。在SelO的C-末端区域鉴定出CxxU基序。当细胞用过氧化氢处理时，该蛋白在HEK 293 T细胞中以时间和浓度依赖性方式可逆地氧化。该处理导致形成瞬时的88 kDa含SelO的复合物。该复合物的形成增强了取代CxxU基序与SxxC，但取消时，它被取代与SxxS，这表明硒与另一种蛋白质通过其Sec残基的氧化还原相互作用。SelO定位于线粒体并在小鼠组织中表达。缺硒对该基因表达的影响较小，提示该基因具有较高的补硒优先性。综上所述，这些结果表明，SelO是一个氧化还原活性的线粒体硒蛋白。
Selenoproteins exhibit diverse biological functions, most of which are associated with redox control. However, the functions of approximately half of mammalian selenoproteins are not known. One such protein is Selenoprotein O (SelO), the largest mammalian selenoprotein with orthologs found in a wide range of organisms, including bacteria and yeast. Here, we report characterization of mammalian SelO. Expression of this protein could be verified in HEK 293T cells by metabolic labeling of cells with 75Se, and it was abolished when selenocysteine was replaced with serine. A CxxU motif was identified in the C-terminal region of SelO. This protein was reversibly oxidized in a time- and concentration-dependent manner in HEK 293T cells when cells were treated with hydrogen peroxide. This treatment led to the formation of a transient 88 kDa SelO-containing complex. The formation of this complex was enhanced by replacing the CxxU motif with SxxC, but abolished when it was replaced with SxxS, suggesting a redox interaction of SelO with another protein through its Sec residue. SelO was localized to mitochondria and expressed across mouse tissues. Its expression was little affected by selenium deficiency, suggesting it has a high priority for selenium supply. Taken together, these results show that SelO is a redox-active mitochondrial selenoprotein.
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DOI：
10.1016/j.bbagen.2009.04.014
发表时间：
2009-11
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Lee BC;Dikiy A;Kim HY;Gladyshev VN
通讯作者：
Gladyshev VN
影响因子：
56.9
作者：
Kryukov, GV;Castellano, S;Gladyshev, VN
通讯作者：
Gladyshev, VN
DOI：
10.1073/pnas.73.8.2659
发表时间：
1976-01-01
影响因子：
11.1
作者：
CONE, JE;MARTINDELRIO, R;STADTMAN, TC
通讯作者：
STADTMAN, TC
DOI：
10.1016/s0006-291x(05)80984-2
发表时间：
1992-05-29
影响因子：
3.1
作者：
HILL, KE;LYONS, PR;BURK, RF
通讯作者：
BURK, RF
DOI：
10.1073/pnas.0610683104
发表时间：
2007-05-08
影响因子：
11.1
作者：
Novoselov, Sergey V.;Lobanov, Alexey V.;Gladyshev, Vadim N.
通讯作者：
Gladyshev, Vadim N.