CD30 expression reveals that culture adaptation of human embryonic stem cells can occur through differing routes.
CD30 expression reveals that culture adaptation of human embryonic stem cells can occur through differing routes.
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DOI：
10.1002/stem.41
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发表时间：
2009-05
期刊：
影响因子：
5.2
通讯作者：
Andrews, Peter W.
中科院分区：
文献类型：
--
作者：
Harrison, Neil J.;Barnes, James;Jones, Mark;Baker, Duncan;Gokhale, Paul J.;Andrews, Peter W.
关键词：
人类胚胎干细胞经历适应性变化，可在体外长时间培养后增加其生长能力。这通常与非随机核型变化有关，通常涉及染色体12、17和x的遗传物质扩增。最近的一项研究表明，核型异常细胞可以通过CD30的表达来识别，CD30赋予细胞凋亡的抗性。我们现在研究了CD30在人胚胎干细胞系H7核型正常和异常亚系中的表达和凋亡，但我们的结果与之前观察到的相反。在该细胞系中，CD30的表达不能区分正常细胞和异常细胞，也不能保护异常细胞免于凋亡。这些数据表明，文化适应可以通过多种机制发生。
Human embryonic stem cells undergo adaptive changes that can increase their growth capacity upon prolonged culture in vitro. This is frequently associated with nonrandom karyotypic changes, commonly involving amplification of genetic material from chromosomes 12, 17, and X. A recent study suggested that the karyotypically abnormal cells can be identified by their expression of CD30, which confers resistance to apoptosis. We have now investigated CD30 expression and apoptosis in karyotypically normal and abnormal sublines of the human ES cell line, H7, but our results were contrary to those previously observed. In this cell line, CD30 expression did not segregate the normal and abnormal cells, and abnormal cells were not protected from apoptosis. These data suggest that culture adaptation can occur through a variety of mechanisms.
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