The use of ball pits and playpens in laboratory Lister Hooded male rats induces ultrasonic vocalisations indicating a more positive affective state and can reduce the welfare impacts of aversive procedures.
The use of ball pits and playpens in laboratory Lister Hooded male rats induces ultrasonic vocalisations indicating a more positive affective state and can reduce the welfare impacts of aversive procedures.
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DOI：
10.1177/00236772211065920
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发表时间：
2022-08
影响因子：
2.4
通讯作者：
中科院分区：
文献类型：
--
作者：
科学的进步和质量依赖于在实验设计、执行、分析和重现性方面稳健和不偏不倚的研究。在临床前研究中，更好地了解动物的情绪并改进它们的饲养、饲养和处理是在实验室环境中提供良好动物福利的重要目标，而实验室环境是严格的研究质量的基础。在动物身上诱导积极的情绪状态是它们幸福的关键组成部分，一种方法是增加它们的环境复杂性，例如使用老鼠的球坑或游乐场。在这项研究中，我们记录了动物暴露在球坑和游戏围栏中的50 khz超声波发声(USV)。我们之前已经证明，50 khz USV提供了动物情绪状态的分级和可量化的衡量标准，这里发现，接近球坑和游戏围栏会增加50 khz USV，表明更积极的情感状态。使用我们的情感偏差测试(ABT)，我们接下来量化了动物对厌恶干预的情绪反应，以及这种反应是否可以通过进入儿童围栏来减弱。与在家中经历药物的动物相比，游戏围栏的暴露完全缓解了引发焦虑的药物引起的负面情绪状态。总而言之，这些发现表明，球坑和活动围栏提供了一种简单而有效的方法来改善实验室大鼠的福利，并减少它们因居住条件和轻微的、令人厌恶的程序而遭受的累积痛苦。
The advancement and quality of science rely on research that is robust and unbiased in its experimental design, execution, analysis, and reproducibility. In preclinical research, a better understanding of animal emotions and refinement of their husbandry, housing, and handling are important goals in providing good animal welfare in a laboratory setting which underpins rigorous research quality. Induction of positive emotional state in animals is a key component of their well-being, and one approach is to increase their environmental complexity using, for example, ball pits or playpens in rats. In this study, we recorded 50 kHz ultrasonic vocalisations (USVs) during animals’ exposure to the ball pit and playpen. We have previously shown that 50 kHz USVs provide a graded and quantifiable measure of an animal’s emotional state, and here find that access to the ball pit and playpen increases 50 kHz USVs, indicative of a more positive affective state. Using our affective bias test (ABT) we next quantified the animals’ emotional response to an aversive intervention and whether this could be attenuated by access to a playpen. The playpen exposure completely mitigated the negative affective state induced by an anxiogenic drug when compared with animals who experienced the drug in the home cage. Together, these findings suggest ball pits and playpens provide a simple and effective method to improve the welfare of laboratory rats and reduce the cumulative suffering they experience from their housing conditions and minor, aversive procedures.
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影响因子：
2.5
作者：
Van Der Harst, JE;Fermont, PCJ;Spruijt, BM
通讯作者：
Spruijt, BM
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通讯作者：
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