Molecular epidemiology, virulence determinants and antimicrobial resistance of Campylobacter spreading in retail chicken meat in Central China.
Molecular epidemiology, virulence determinants and antimicrobial resistance of Campylobacter spreading in retail chicken meat in Central China.
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DOI：
10.1186/s13099-016-0132-2
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发表时间：
2016
期刊：
影响因子：
4.2
通讯作者：
Shao H
中科院分区：
文献类型：
--
作者：
Zhang T;Luo Q;Chen Y;Li T;Wen G;Zhang R;Luo L;Lu Q;Ai D;Wang H;Shao H
弯曲杆菌属是引起人类细菌性胃肠炎的主要食源性致病菌。受污染的鸡肉产品被认为是弯曲杆菌传播给人类的主要媒介。本研究调查了华中地区零售鸡肉中弯曲杆菌的流行情况，并利用分子生物学方法对分离株进行了进一步的鉴定和耐药性检测。本署在2014年4月至2015年4月期间，共抽取302个鸡只样本进行测试。弯曲杆菌污染水平采用最大可能数PCR（MPN PCR）计数。弯曲菌阳性率为17.2%（52/302），阳性标本细菌数为3.6 ~ 360 MPN/g。从阳性标本中分离出52株弯曲菌，其中空肠弯曲菌40株，结肠弯曲菌12株。为了研究菌株的遗传多样性，应用多位点序列分型（MLST）技术，确定了23个序列类型（ST）属于7个克隆复合体（CC）和未分配。其中，C.空肠的CC为CC-353和CC-464，C.大肠杆菌CC-828和CC-1150。耐药性分析显示，所有分离株均对诺氟沙星和环丙沙星耐药。结果表明，在分离株中检测到的毒力相关基因数为23个。jejuni分离株16 ~ 21株，而绝大多数C.大肠埃希菌12 ~ 16株。所有菌株均检测到毒力相关基因flaA、flgB、flgE 2、fliM、fliY和cadF。然而，VirB 11在任何分离株中均未检测到。据我们所知，这是第一次报告弯曲菌在中国中部零售鸡肉污染水平和分子生物学特性，显示出较高的遗传多样性和显着的抗生素耐药性。本研究为零售鸡肉产品中弯曲杆菌污染的风险评估和评价提供了科学依据。本文的在线版本（doi：10.1186/s13099-016-0132-2）包含补充材料，可供授权用户使用。
Campylobacter species are the major food-borne pathogens which could cause bacterial gastroenteritis in humans. Contaminated chicken products have been recognized as the primary vehicles of Campylobacter transmission to human beings. In this study, the prevalence of Campylobacter in retail chicken meat in Central China was investigated, and the isolates were further characterized using molecular approaches and tested for antibiotic resistance. A total of 302 chicken samples purchased from April 2014 to April 2015 were tested. The level of Campylobacter contamination was enumerated by most probable number-PCR (MPN-PCR). The Campylobacter positive rate was 17.2% (52/302), with bacterial count varying from 3.6 to 360 MPN/g in positive samples. A total of 52 Campylobacter strains, including 40 Campylobacter jejuni and 12 Campylobacter coli, were isolated from the positive samples. To examine the genetic diversity of the isolates, multilocus sequence typing (MLST) technology was applied, which identified 23 sequence types (STs) belonging to seven clonal complexes (CCs) and unassigned. Among them, the dominant CCs of C. jejuni included CC-353 and CC-464, and the dominant CCs of C. coli were CC-828 and CC-1150. Antibiotic resistance analysis showed that all of the isolates were resistant to norfloxacin and ciprofloxacin. 23 virulence-associated genes were tested in the isolates, which showed that the number of virulence-associated genes detected in the C. jejuni isolates ranged from 16 to 21, while in most of the C. coli isolates ranged from 12 to 16. Virulence-associated genes, flaA, flgB, flgE2, fliM, fliY and cadF were detected in all isolates. VirB11, however, was not detected in any of the isolates. To the best of our knowledge, this is the first report on the contamination level and molecular biological features of Campylobacter strains in retail chicken meat in Central China, which showed high genetic diversity and remarkable antibiotic resistance. This study provided scientific data for the risk assessment and evaluation of Campylobacter contamination in retail chicken products. The online version of this article (doi:10.1186/s13099-016-0132-2) contains supplementary material, which is available to authorized users.
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影响因子：
11.8
作者：
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