Infant gut microbiome is enriched with Bifidobacterium longum ssp. infantis in Old Order Mennonites with traditional farming lifestyle.
Infant gut microbiome is enriched with Bifidobacterium longum ssp. infantis in Old Order Mennonites with traditional farming lifestyle.
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作者：
在传统的单一家庭农场长大与学龄儿童预防哮喘有关，但预防特应性疾病早期表现的机制在很大程度上是未知的。我们试图确定农村Old Order Mennonite （OOM）低风险婴儿和纽约州罗切斯特市城市/郊区特应性疾病高风险婴儿的肠道微生物组和代谢组组成。在65对OOM和39对Rochester母婴队列中，通过16S rRNA基因测序对101个婴儿粪便和61个人乳样本进行微生物组组成和qPCR对双歧杆菌和长双歧杆菌进行定量分析。婴儿（B. infantis），人乳寡糖（HMOs）的消费者。测定了34份粪便和24份人乳样品的脂肪酸含量。通过电话评估3岁儿童特应性疾病的诊断和症状。在平均2个月大时，与罗切斯特婴儿相比，OOM的粪便中富含双歧杆菌科、梭菌科和气球菌科。B.婴儿更为丰富（p<0.001）和普遍，在70%的OOM中检测到，而在Rochester婴儿中检测到的比例为21% （p<0.001）。婴儿b型梭菌定植的粪便具有较高的乳酸水平和几种中至长/奇链FAs。相比之下，人乳富含一组独特的脂肪酸，包括丁酸盐。6.5%的OOM儿童和35%的Rochester儿童报告了特应性疾病（p<0.001）。在患特应性疾病风险较低的东部地区，婴儿b型结核菌定植率较高，与发展中国家相似。
Growing up on traditional, single-family farms is associated with protection against asthma in school-age, but the mechanisms against early manifestations of atopic disease are largely unknown. We sought determine the gut microbiome and metabolome composition in rural Old Order Mennonite (OOM) infants at low risk and Rochester, NY urban/suburban infants at high risk for atopic diseases. In a cohort of 65 OOM and 39 Rochester mother-infant pairs, 101 infant stool and 61 human milk samples were assessed by 16S rRNA gene sequencing for microbiome composition and qPCR to quantify Bifidobacterium spp. and B. longum ssp. infantis (B. infantis), a consumer of human milk oligosaccharides (HMOs). Fatty acids (FAs) were analyzed in 34 stool and human 24 milk samples. Diagnoses and symptoms of atopic diseases by 3 years of age were assessed by telephone. At a median age of 2 months, stool was enriched with Bifidobacteriaceae, Clostridiaceae and Aerococcaceae in the OOM compared to Rochester infants. B. infantis was more abundant (p<0.001) and prevalent, detected in 70% of OOM compared to 21% of Rochester infants (p<0.001). Stool colonized with B. infantis had higher levels of lactate and several medium- to long/odd-chain FAs. In contrast, human milk was enriched with a distinct set of FAs including butyrate. Atopic diseases were reported in 6.5% of OOM and 35% of Rochester children (p<0.001). A high rate of B. infantis colonization, similar to that seen in developing countries, is found in the OOM at low risk for atopic diseases.
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DOI：
10.1126/science.1198469
发表时间：
2011-01-21
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Atarashi K;Tanoue T;Shima T;Imaoka A;Kuwahara T;Momose Y;Cheng G;Yamasaki S;Saito T;Ohba Y;Taniguchi T;Takeda K;Hori S;Ivanov II;Umesaki Y;Itoh K;Honda K
通讯作者：
Honda K
影响因子：
8
作者：
Cait, A.;Hughes, M. R.;Mohn, W. W.
通讯作者：
Mohn, W. W.
影响因子：
2.6
作者：
Gritz EC;Bhandari V
通讯作者：
Bhandari V
影响因子：
17.1
作者：
Arrieta, Marie-Claire;Stiemsma, Leah T.;Finlay, B. Brett
通讯作者：
Finlay, B. Brett
影响因子：
4.4
作者：
Dzidic, Majda;Mira, Alex;Carmen Collado, Maria
通讯作者：
Carmen Collado, Maria