Biased cellular locations of tandem repeat antigens in African trypanosomes.
Biased cellular locations of tandem repeat antigens in African trypanosomes.
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DOI：
10.1016/j.bbrc.2011.01.048
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发表时间：
2011-02-18
影响因子：
3.1
通讯作者：
Carter D
中科院分区：
文献类型：
--
作者：
Goto Y;Duthie MS;Kawazu S;Inoue N;Carter D
布氏锥虫亚种在人类和动物中引起非洲锥虫病。这些寄生虫具有编码具有大串联重复（TR）结构域的蛋白质的基因，就像其他锥虫寄生虫一样。我们以前已经证明婴儿利什曼原虫和T. cruzi通常是B细胞反应的目标。然而，非洲锥虫对抗体介导的免疫敏感，并且寄生虫在细胞表面上具有这种B细胞抗原可能是有害的。这里我们展示了T.布鲁氏菌亚种也具有抗原性：这些寄生虫的重组TR蛋白通过ELISA检测来自感染寄生虫的小鼠的血清中的抗体。氨基酸序列分析表明，该蛋白与利什曼原虫TR蛋白或T. cruzi，预测的信号肽，跨膜结构域和GPI锚定信号在T.布氏菌TR蛋白质组的蛋白质含量明显低于整个蛋白质组。许多T。布氏杆菌TR蛋白在物种中是特异性的，或者仅在密切相关的物种中是保守的，对于L. major和T.克鲁兹这些结果表明，尽管他们有一些共同的特点，这种丰富的大TR结构域和免疫优势，TR基因之间的锥虫寄生虫独立进化。
Trypanosoma brucei subspecies cause African trypanosomiasis in humans and animals. These parasites possess genes encoding proteins with large tandem repeat (TR) domains as do the other trypanosomatid parasites. We have previously demonstrated that TR protein of Leishmania infantum and T. cruzi are often targets of B-cell responses. However, African trypanosomes are susceptible to antibody-mediated immunity, and it may be detrimental for the parasites to have such B-cell antigens on the cell surface. Here we show TR proteins of T. brucei subspecies are also antigenic: recombinant TR proteins of these parasites detect antibodies in sera from mice infected with the parasites by ELISA. Analysis of amino acid sequences revealed that, different from TR proteins of Leishmania species or T. cruzi, the presence of predicted signal peptides, trans-membrane domains and GPI anchor signals in T. brucei TR proteins are significantly lower than those of the whole proteome. Many of the T. brucei TR proteins are specific in the species or conserved only in the closely related species, as is the same case for L. major and T. cruzi. These results suggest that, despite their sharing some common characteristics, such abundance in large TR domains and immunological dominance, TR genes have evolved independently among the trypanosomatid parasites.
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影响因子：
2.6
作者：
Goto, Yasuyuki;Howard, Randall F.;Bhatia, Ajay;Trigo, Joelma;Nakatani, Maria;Netto, Eduardo M.;Reed, Steven G.
通讯作者：
Reed, Steven G.
影响因子：
3.1
作者：
Goto, Yasuyuki;Carter, Darrick;Reed, Steven G.
通讯作者：
Reed, Steven G.
影响因子：
9.4
作者：
Kuboki, N;Inoue, N;Igarashi, I
通讯作者：
Igarashi, I
DOI：
10.1073/pnas.93.9.4131
发表时间：
1996-04-30
影响因子：
11.1
作者：
Madoff, LC;Michel, JL;Kasper, DL
通讯作者：
Kasper, DL
影响因子：
56.9
作者：
Ivens, AC;Peacock, CS;Myler, PJ
通讯作者：
Myler, PJ