Distinct antigen recognition pattern during zoonotic visceral leishmaniasis in humans and dogs.
Distinct antigen recognition pattern during zoonotic visceral leishmaniasis in humans and dogs.
复制标题
DOI：
10.1016/j.vetpar.2008.10.097
复制
发表时间：
2009-03-23
影响因子：
2.6
通讯作者：
Reed, Steven G.
中科院分区：
文献类型：
--
作者：
Goto, Yasuyuki;Howard, Randall F.;Bhatia, Ajay;Trigo, Joelma;Nakatani, Maria;Netto, Eduardo M.;Reed, Steven G.
婴儿利什曼原虫是南美洲和地中海地区地方性人畜共患内脏利什曼病(VL)的病原体。狗是这些地区婴儿利什曼原虫的主要宿主，控制狗的疾病可能对人类疾病产生重大影响。虽然由于婴儿利什曼原虫的感染，狗与人类有许多共同的VL症状，但它们也表现出一些独特的临床表现，通常是内脏和皮肤利什曼病的结合，这表明狗和人类的疾病发展机制不同。在这里，我们比较了VL犬和人对各种确定的利什曼病毒抗原的抗体反应。寄生虫裂解物和K39是两种最常用的VL血清学诊断抗原，在VL患者中检测到的抗体水平最高，但这些抗原的识别模式在宿主之间是不同的。在测试的其他确定的抗原中，LmSTI1和CPB分别在狗和人类中检测到较高水平的抗体。这些结果表明，在VL期间，人和狗在抗原识别模式上存在差异；我们推断，在为人和狗开发疫苗和诊断时，可能需要使用不同的策略。此外，我们还显示了几种抗原的抗体滴度与犬VL期间临床症状的严重程度之间的相关性。
Leishmania infantum is a causative agent of endemic zoonotic visceral leishmaniasis (VL) in regions of South America and the Mediterranean. Dogs are the major reservoirs for Leishmania infantum in these regions, and control of disease in dogs could have a significant impact on human disease. Although dogs share many symptoms of VL with humans as a result of L. infantum infection, they also show some unique clinical manifestations, which are often a combination of visceral and cutaneous leishmaniasis, suggesting different mechanisms of disease development in dogs and humans. Here, we compare antibody responses of dogs and humans with VL to various defined leishmanial antigens. Parasite lysate and K39, the two most commonly used antigens for serodiagnosis of VL, detected the highest levels of antibodies in both humans and dogs with VL, whereas the recognition patterns of these antigens were distinct between the hosts. Among other defined antigens tested, LmSTI1 and CPB detected higher levels of antibodies in dogs and humans, respectively. These results indicate there is a difference between humans and dogs in antigen recognition patterns during VL; we infer that different strategies may need to be used in development of vaccines and diagnostics for humans and for dogs. In addition, we show a correlation between antibody titers to several antigens and severity of clinical symptoms during canine VL.
登录
查看更多内容
DOI：
10.1128/cdli.4.5.530-535.1997
发表时间：
1997-09-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
--
作者：
Ghedin, E;Zhang, WW;Matlashewski, G
通讯作者：
Matlashewski, G
影响因子：
5.5
作者：
Ghosh, A;Zhang, WW;Matlashewski, G
通讯作者：
Matlashewski, G
影响因子：
6.4
作者：
Badaro, R;Benson, D;Reed, SG
通讯作者：
Reed, SG
影响因子：
5.5
作者：
Goto, Yasuyuki;Bogatzki, Lisa Y.;Reed, Steven G.
通讯作者：
Reed, Steven G.
影响因子：
5.5
作者：
Rafati, S;Zahedifard, F;Nazgouee, F
通讯作者：
Nazgouee, F