Systemic Injection of Substance P Promotes Murine Calvarial Repair Through Mobilizing Endogenous Mesenchymal Stem Cells.
Systemic Injection of Substance P Promotes Murine Calvarial Repair Through Mobilizing Endogenous Mesenchymal Stem Cells.
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全身注射 P 物质通过动员内源性间充质干细胞促进小鼠颅骨修复
DOI：
10.1038/s41598-018-31414-5
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发表时间：
2018-08-29
影响因子：
4.6
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Zhang Y;An S;Hao J;Tian F;Fang X;Wang J
颅面缺损是牙科诊所面临的一个关键问题，它对患者的生活质量有巨大影响。基于间充质干细胞的疗法已成为组织缺损修复的一种有前景的方法。然而，间充质干细胞移植后存活率降低仍然是该领域的一个主要问题，这阻碍了再生的效果。最近，动员内源性间充质干细胞用于组织再生的机制受到了越来越多的关注，因为它不需要外源性细胞移植。本研究的主要目的是确认静脉注射P物质在临界尺寸骨缺损动物中动员内源性CD45 - CD11b - CD29 + 间充质干细胞的作用，并研究P物质对颅骨修复的影响。流式细胞术分析显示，静脉注射P物质促进了骨缺损后内源性CD45 - CD11b - CD29 + 间充质干细胞的动员。此外，微型计算机断层扫描（Micro - CT）显示静脉注射P物质改善了颅骨修复的效果。进一步地，我们发现全身注射P物质减轻了炎症并提高了局部移植的绿色荧光蛋白阳性（GFP +）间充质干细胞的存活率。我们的研究结果表明，P物质及其动员的CD45 - CD11b - CD29 + 间充质干细胞通过调节炎症条件和提高局部移植细胞的存活率，有助于改善颅骨缺损修复。
Craniofacial defect is a critical problem in dental clinic, which has a tremendous impact on patients’ quality of life. Mesenchymal stem cell-based therapy has emerged as a promising approach for tissue defect repair. However, reduced survival after mesenchymal stem cells (MSCs) transplantation remains as a major problem in this area, which hampers the outcome of regeneration. Recently, the mechanism to mobilize endogenous MSCs for tissue regeneration has received increasing attentions, as it does not require exogenous cell transplantation. The primary goal of this study was to confirm the role of intravenous substance P in mobilizing endogenous CD45−CD11b−CD29+ MSCs in critical-sized bone defect animals and to investigate the effects of substance P on calvarial bone repair. Flow cytometry analyses revealed that intravenous substance P promoted the mobilization of endogenous CD45−CD11b−CD29+ MSCs after bone defect. In addition, Micro-CT showed that intravenous substance P improved the outcomes of calvarial bone repair. Furthermore, we discovered that systemic injection of substance P attenuated inflammation and enhanced the survival of the local-transplanted GFP+ MSCs. Our findings suggested that substance P together with its mobilized CD45−CD11b−CD29+ MSCs helped improve calvarial defect repair through regulating inflammatory conditions and promoting the survival of local-transplanted cells.
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影响因子：
3.6
作者：
Hamou C;Callaghan MJ;Thangarajah H;Chang E;Chang EI;Grogan RH;Paterno J;Vial IN;Jazayeri L;Gurtner GC
通讯作者：
Gurtner GC
影响因子：
6.4
作者：
Mao, Jeremy J.;Stosich, Michael S.;Ahn, Jeffrey
通讯作者：
Ahn, Jeffrey
影响因子：
12.4
作者：
Oh, Joo Youn;Lee, Ryang Hwa;Prockop, Darwin J.
通讯作者：
Prockop, Darwin J.
影响因子：
4.5
作者：
Zachar L;Bačenková D;Rosocha J
通讯作者：
Rosocha J
影响因子：
2.6
作者：
Awaya, N;Rupert, K;Torok-Storb, B
通讯作者：
Torok-Storb, B