Polyploid genome of Camelina sativa revealed by isolation of fatty acid synthesis genes.
Polyploid genome of Camelina sativa revealed by isolation of fatty acid synthesis genes.
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DOI：
10.1186/1471-2229-10-233
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发表时间：
2010-10-27
影响因子：
5.3
通讯作者：
Kiser J
中科院分区：
文献类型：
--
作者：
Hutcheon C;Ditt RF;Beilstein M;Comai L;Schroeder J;Goldstein E;Shewmaker CK;Nguyen T;De Rocher J;Kiser J
亚麻荠是油菜科的一种油料作物，由于其生物燃料应用的潜力而引起了人们的新兴趣。很少有人了解C的性质。sativa基因组。对脂肪酸生物合成途径中的两个基因--脂肪酸去饱和酶（FAD）2和脂肪酸延伸酶（FAE）1进行了研究，揭示了C. sativa基因组In C. sativa中，Southern分析表明存在FAD 2和FAE 1的三个拷贝以及LFY，LFY是其他物种中已知的单拷贝基因。CsFAD 2和CsFAE 1的所有三个拷贝都在发育中的种子中表达，序列比对显示存在先前描述的保守位点，表明这两个基因的所有三个拷贝都可能具有功能。CsFAD 2的下游区域和CsFAE 1的上游区域显示与拟南芥基因组共线性。此外，在454个测序分析中，6个预测的单拷贝基因观察到3种表达单倍型，流式细胞术结果表明，C. sativa是二倍体亚麻荠属近缘种的大约三倍。系统发育分析进一步支持了重复的历史，并表明C。sativa和C. microcarpa可能共享一个父母的基因组。有令人信服的证据表明，C。Sativa基因组，包括一个更大的染色体数目和三倍大的测量基因组大小比其他亚麻荠属亲属，三个分离的FAD 2，FAE 1，和KCS 17-FAE 1基因间区域的副本，和三个表达的单倍型观察到的六个预测的单拷贝基因。基于这些结果，我们提出C. sativa被认为是异源六倍体。脂肪酸合成途径基因的表征将允许未来操纵这种新兴生物燃料作物的油组成;然而，油组成的靶向操纵和C. sativa应该考虑，并在可能的情况下利用，多倍体的含义。
Camelina sativa, an oilseed crop in the Brassicaceae family, has inspired renewed interest due to its potential for biofuels applications. Little is understood of the nature of the C. sativa genome, however. A study was undertaken to characterize two genes in the fatty acid biosynthesis pathway, fatty acid desaturase (FAD) 2 and fatty acid elongase (FAE) 1, which revealed unexpected complexity in the C. sativa genome. In C. sativa, Southern analysis indicates the presence of three copies of both FAD2 and FAE1 as well as LFY, a known single copy gene in other species. All three copies of both CsFAD2 and CsFAE1 are expressed in developing seeds, and sequence alignments show that previously described conserved sites are present, suggesting that all three copies of both genes could be functional. The regions downstream of CsFAD2 and upstream of CsFAE1 demonstrate co-linearity with the Arabidopsis genome. In addition, three expressed haplotypes were observed for six predicted single-copy genes in 454 sequencing analysis and results from flow cytometry indicate that the DNA content of C. sativa is approximately three-fold that of diploid Camelina relatives. Phylogenetic analyses further support a history of duplication and indicate that C. sativa and C. microcarpa might share a parental genome. There is compelling evidence for triplication of the C. sativa genome, including a larger chromosome number and three-fold larger measured genome size than other Camelina relatives, three isolated copies of FAD2, FAE1, and the KCS17-FAE1 intergenic region, and three expressed haplotypes observed for six predicted single-copy genes. Based on these results, we propose that C. sativa be considered an allohexaploid. The characterization of fatty acid synthesis pathway genes will allow for the future manipulation of oil composition of this emerging biofuel crop; however, targeted manipulations of oil composition and general development of C. sativa should consider and, when possible take advantage of, the implications of polyploidy.
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影响因子：
3.4
作者：
Duarte, Jill M.;Wall, P. Kerr;dePamphilis, Claude W.
通讯作者：
dePamphilis, Claude W.
影响因子：
5.9
作者：
Bernardo, A;Howard-Hildige, R;Leahy, JJ
通讯作者：
Leahy, JJ
影响因子：
3.1
作者：
Belo, Andre;Zheng, Peizhong;Rafalski, Antoni
通讯作者：
Rafalski, Antoni
DOI：
10.1111/j.1095-8312.2004.00337.x
发表时间：
2004-08-01
影响因子：
1.9
作者：
Brochmann, C;Brysting, AK;Elven, R
通讯作者：
Elven, R
影响因子：
3.1
作者：
Gehringer, A.;Friedt, W.;Snowdon, R. J.
通讯作者：
Snowdon, R. J.