A timer-actuated immunoassay cassette for detecting molecular markers in oral fluids.
A timer-actuated immunoassay cassette for detecting molecular markers in oral fluids.
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DOI：
10.1039/b814322f
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发表时间：
2009-03-21
期刊：
影响因子：
6.1
通讯作者：
Bau HH
中科院分区：
文献类型：
--
作者：
Liu C;Qiu X;Ongagna S;Chen D;Chen Z;Abrams WR;Malamud D;Corstjens PL;Bau HH
设计、构建并测试了一种便宜的手持式护理点一次性独立免疫测定盒，其包括用于泵送的空气袋、计量室、试剂储存室、混合器和侧向流动条。以连续流动形式进行测定。用上转换磷光体（UCP）报告颗粒促进检测。各种试剂的自动、及时泵送由弹簧计时器驱动。通过检测唾液样本中的HIV抗体证明了盒的实用性，并进一步用非传染性半抗原化DNA测定法进行了评估。试剂盒与量油尺相比具有几个优点，例如样品预处理、试剂的集成存储以及减少操作员错误和培训的自动化操作。本文所述的盒和致动器可以容易地扩展以在护理点检测体液和其他流体中的其他疾病的生物标志物。这一制度特别适合于资金和训练有素的人员短缺的资源贫乏国家。
An inexpensive, hand-held, point-of-care, disposable, self-contained, immunoassay cassette comprised of air pouches for pumping, a metering chamber, reagents storage chambers, a mixer, and a lateral flow strip was designed, constructed, and tested. The assay was carried out in a consecutive flow format. The detection was facilitated with up-converting, phosphor (UCP) reporter particles. The automated, timely pumping of the various reagents was driven by a spring-loaded timer. The utility of the cassette was demonstrated by detecting antibodies to HIV in saliva samples and further evaluated with a non-contagious, haptenized DNA assay. The cassette has several advantages over dip sticks such as sample preprocessing, integrated storage of reagents, and automated operation that reduces operator errors and training. The cassette and actuator described herein can readily be extended to detect biomarkers of other diseases in body fluids and other fluids at the point of care. The system is particularly suitable for resource poor countries, where funds and trained personnel are in short supply.
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影响因子：
6.1
作者：
Cesaro-Tadic, S;Dernick, G;Delamarche, E
通讯作者：
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影响因子：
7.4
作者：
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通讯作者：
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影响因子：
2.9
作者：
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影响因子：
9.4
作者：
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通讯作者：
van Dam, Govert J.
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影响因子：
4.1
作者：
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