Metagenomic analysis of the airborne environment in urban spaces.
Metagenomic analysis of the airborne environment in urban spaces.
复制标题
城市空间中空降环境的宏基因组分析。
DOI:
10.1007/s00248-014-0517-z
复制
发表时间:
2015-02
影响因子:
3.6
通讯作者:
Jaing, Crystal J.
中科院分区:
文献类型:
--
作者:
Be, Nicholas A.;Thissen, James B.;Fofanov, Viacheslav Y.;Allen, Jonathan E.;Rojas, Mark;Golovko, George;Fofanov, Yuriy;Koshinsky, Heather;Jaing, Crystal J.
The organisms in aerosol microenvironments, especially densely populated urban areas, are relevant to maintenance of public health and detection of potential epidemic or biothreat agents. To examine aerosolized microorganisms in this environment, we performed sequencing on the material from an urban aerosol surveillance program. Whole metagenome sequencing was applied to DNA extracted from air filters obtained during periods from each of the four seasons. The composition of bacteria, plants, fungi, invertebrates, and viruses demonstrated distinct temporal shifts. Bacillus thuringiensis serovar kurstaki was detected in samples known to be exposed to aerosolized spores, illustrating the potential utility of this approach for identification of intentionally introduced microbial agents. Together, these data demonstrate the temporally dependent metagenomic complexity of urban aerosols and the potential of genomic analytical techniques for biosurveillance and monitoring of threats to public health. The online version of this article (doi:10.1007/s00248-014-0517-z) contains supplementary material, which is available to authorized users.
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影响因子:
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通讯作者:
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作者:
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通讯作者:
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