Distinct fecal and oral microbiota composition in human type 1 diabetes, an observational study.
Distinct fecal and oral microbiota composition in human type 1 diabetes, an observational study.
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DOI：
10.1371/journal.pone.0188475
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Nieuwdorp M
中科院分区：
文献类型：
--
作者：
de Groot PF;Belzer C;Aydin Ö;Levin E;Levels JH;Aalvink S;Boot F;Holleman F;van Raalte DH;Scheithauer TP;Simsek S;Schaap FG;Olde Damink SWM;Roep BO;Hoekstra JB;de Vos WM;Nieuwdorp M
1型糖尿病（T1D）的环境因素仍然是未知的。动物和人类研究都表明，粪便微生物群组成改变、短链脂肪酸（SCFA）产生受损和T1D发作之间存在关联。然而，缺乏长期T1D成人与健康对照（HC）的SCFA和粪便和口腔微生物群的观察证据。我们纳入了53例无并发症或药物治疗的T1D患者和50例年龄、性别和BMI匹配的HC患者。测量口腔和粪便微生物群、粪便和血浆SCFA水平、肠道炎症标志物（粪便伊加和钙卫蛋白）和低度全身性炎症标志物。与HC相比，T1D的口腔微生物群（例如链球菌丰度）显著不同。粪便分析显示T1D中产生丁酸的种类减少，丁酰辅酶A转移酶基因减少。此外，T1D患者的乙酸盐和丙酸盐血浆水平较低，粪便SCFA相似。最后，粪便菌株Christensenella和Subdoligranulum与血糖控制、炎症参数和SCFA相关。我们得出结论，T1D患者具有不同数量的肠道SCFA（丁酸盐）生产者和不同的血浆乙酸盐和丙酸盐水平。未来的研究应该解开因果关系，以及补充SCFA生产细菌或单独SCFA是否可以在T1D中产生疾病修饰作用。
Environmental factors driving the development of type 1 diabetes (T1D) are still largely unknown. Both animal and human studies have shown an association between altered fecal microbiota composition, impaired production of short-chain fatty acids (SCFA) and T1D onset. However, observational evidence on SCFA and fecal and oral microbiota in adults with longstanding T1D vs healthy controls (HC) is lacking. We included 53 T1D patients without complications or medication and 50 HC matched for age, sex and BMI. Oral and fecal microbiota, fecal and plasma SCFA levels, markers of intestinal inflammation (fecal IgA and calprotectin) and markers of low-grade systemic inflammation were measured. Oral microbiota were markedly different in T1D (eg abundance of Streptococci) compared to HC. Fecal analysis showed decreased butyrate producing species in T1D and less butyryl-CoA transferase genes. Also, plasma levels of acetate and propionate were lower in T1D, with similar fecal SCFA. Finally, fecal strains Christensenella and Subdoligranulum correlated with glycemic control, inflammatory parameters and SCFA. We conclude that T1D patients harbor a different amount of intestinal SCFA (butyrate) producers and different plasma acetate and propionate levels. Future research should disentangle cause and effect and whether supplementation of SCFA-producing bacteria or SCFA alone can have disease-modifying effects in T1D.
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影响因子：
15.5
作者：
Endesfelder D;Engel M;Davis-Richardson AG;Ardissone AN;Achenbach P;Hummel S;Winkler C;Atkinson M;Schatz D;Triplett E;Ziegler AG;zu Castell W
通讯作者：
zu Castell W
影响因子：
64.8
作者：
Planer JD;Peng Y;Kau AL;Blanton LV;Ndao IM;Tarr PI;Warner BB;Gordon JI
通讯作者：
Gordon JI
影响因子：
120.7
作者：
Dabelea, Dana;Mayer-Davis, Elizabeth J.;Saydah, Sharon;Imperatore, Giuseppina;Linder, Barbara;Divers, Jasmin;Bell, Ronny;Badaru, Angela;Talton, Jennifer W.;Crume, Tessa;Liese, Angela D.;Merchant, Anwar T.;Lawrence, Jean M.;Reynolds, Kristi;Dolan, Lawrence;Liu, Lenna L.;Hamman, Richard F.
通讯作者：
Hamman, Richard F.
广谱抗生素治疗与罹患 1 型糖尿病的儿童： 丹麦全国队列研究》。
影响因子：
3.7
作者：
Clausen TD;Bergholt T;Bouaziz O;Arpi M;Eriksson F;Rasmussen S;Keiding N;Løkkegaard EC
通讯作者：
Løkkegaard EC
影响因子：
4.4
作者：
Louis, Petra;Flint, Harry J.
通讯作者：
Flint, Harry J.