MiR16 as a microRNA marker applied in species identification
MiR16 as a microRNA marker applied in species identification
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MiR16作为microRNA标记应用于物种鉴定
DOI：
10.1016/j.fsigss.2011.09.019
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发表时间：
2011-12
影响因子：
--
通讯作者：
Yiping Hou
中科院分区：
文献类型：
--
作者：
Yan Li;Zheng Wang;Yiping Hou
MicroRNA（miRNAs，长度为18-25个碱基）是一类小的非编码RNA，在转录后水平调控基因表达。miRNA表达模式，包括特定miRNA种类的存在和相对丰度，提供了可用于体液鉴定的细胞和组织特异性信息。最近，两项已发表的研究报道，已经鉴定出许多体液特异性miRNA。然而，他们专注于识别特定的人体体液，而没有考虑物种特异性。本研究选取了血液中高丰度的miR 16作为研究对象，检测了9种动物血液中miR 16的表达丰度（猴、兔、鸡、鸭、牛、羊、狗、SD大鼠和小鼠）使用高特异性茎环逆转录（RT）和高灵敏度水解探针（TaqMan）定量实时聚合酶链反应（qPCR）。结果表明，miR 16在9种动物中差异表达，需要进一步研究，寻找更多的miRNA标记物来区分人血痕和动物血痕。
MicroRNAs (miRNAs, 18–25 bases in length) are small, non-coding RNAs that regulate gene expression at the post-transcriptional level. MiRNAs expression patterns, including presence and relative abundance of particular miRNA species, provide cell- and tissue-specific information that can be used for body fluid identification. Recently, two published studies reported that a number of body fluid-specific miRNAs had been identified. However, they focused on identifying specific human body fluids and did not consider species specificity. In this study, we selected blood high abundance miR16 that be reported it can distinguish venous blood from other body fluids in human to detect the expression abundance in blood of nine animals (monkey, rabbit, chicken, duck, cattle, sheep, dog, SD rat and mouse) using high-specificity stem-loop reverse transcription (RT) and high-sensitivity hydrolysis probes (TaqMan) quantitative real-time polymerase chain reaction (qPCR). The results showed that miR16 is differentially expressed in nine animals, which need to be further studied and additional work remains necessary search for more miRNA markers to identify human blood stain from animal blood stains.
DOI：
10.1016/j.fsigss.2009.08.184
发表时间：
2009-12-01
期刊：
FORENSIC SCIENCE INTERNATIONAL GENETICS SUPPLEMENT SERIES
影响因子：
--
作者：
Hanson, E.;Lubenow, H.;Ballantyne, J.
通讯作者：
Ballantyne, J.
影响因子：
2.1
作者：
Zubakov D;Boersma AW;Choi Y;van Kuijk PF;Wiemer EA;Kayser M
通讯作者：
Kayser M
影响因子：
2.9
作者：
Hanson, Erin K.;Lubenow, Helge;Ballantyne, Jack
通讯作者：
Ballantyne, Jack