FKBP39 Controls the Larval Stage JH Activity and Development in Drosophila melanogaster.
FKBP39 Controls the Larval Stage JH Activity and Development in Drosophila melanogaster.
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FKBP39 控制果蝇幼虫阶段 JH 活动和发育
作者：
两种内分泌激素，蜕皮激素和保幼激素（JH），控制昆虫的发育和繁殖。文献中的一些研究表明，FKBP39 在果蝇中作为转录因子发挥作用并调节 JH 通路。然而，FKBP39 的生理作用仍不清楚。为了确定 FKBP39 在体内的作用，我们首先开发了一种抗体来检查 FKBP39 表达模式，然后检测 fkbp39 突变体中 JH 活性相关的表型，例如蛹化、繁殖和 Kr-h1 表达。我们发现 FKBP39 在幼虫阶段高水平表达并控制 JH 活性。此外，我们发现实时定量PCR（qRT-PCR）中使用最广泛的参考基因rp49在fkbp39突变体中显着下降。这项工作将为 JH 活动和昆虫发育的研究提供有价值的信息。 FK506 结合蛋白 39kD (FKBP39) 定位于细胞核并包含多个功能域。结构分析表明 FKBP39 可能作为转录因子发挥作用并控制保幼激素 (JH) 活性。在这里，我们发现 FKBP39 在前两个幼虫阶段和第三个幼虫阶段早期以高水平表达并定位于脂肪体细胞的核仁中。 fkbp39 突变体在第三幼虫阶段表现出幼虫-蛹转变延迟和 Kr-h1（JH 途径的主要介质）表达增加。此外，fkbp39突变体具有与JH活性无关的生育缺陷。有趣的是，rp49（果蝇中 qRT-PCR 最广泛使用的参考基因）的表达在 fkbp39 突变体中显着降低，表明 FKBP39 可能调节核糖体组装。综上所述，我们的数据证明了 FKBP39 在果蝇中的表达模式和生理作用。
Two endocrine hormones, ecdysone and juvenile hormone (JH), control insect development and reproduction. Some studies in the literature have suggested that FKBP39 functions as a transcriptional factor and regulates the JH pathway in Drosophila. However, the physiological roles of FKBP39 are still elusive. To determine the FKBP39 roles in vivo, we first developed an antibody to check the FKBP39 expression pattern and then detected JH activity-related phenotypes in fkbp39 mutants, such as pupariation, reproduction, and Kr-h1 expression. We found that FKBP39 expresses at a high level and controls JH activity at the larval stage. Moreover, we found that rp49, the most widely used reference gene for Real-time quantitative PCR (qRT-PCR), significantly decreased in the fkbp39 mutant. This work will provide valuable information for studies on JH activity and insect development. FK506-binding protein 39kD (FKBP39) localizes in the nucleus and contains multiple functional domains. Structural analysis suggests that FKBP39 might function as a transcriptional factor and control juvenile hormone (JH) activity. Here, we show that FKBP39 expresses at a high level and localizes in the nucleolus of fat body cells during the first two larval stages and early third larval stage. The fkbp39 mutant displays delayed larval-pupal transition and an increased expression of Kr-h1, the main mediator of the JH pathway, at the early third larval stage. Moreover, the fkbp39 mutant has a fertility defect that is independent of JH activity. Interestingly, the expression of rp49, the most widely used reference gene for qRT-PCR in Drosophila, significantly decreased in the fkbp39 mutant, suggesting that FKBP39 might regulate ribosome assembly. Taken together, our data demonstrate the expression pattern and physiological roles of FKBP39 in Drosophila.
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DOI：
10.1073/pnas.1716897115
发表时间：
2018-01-02
影响因子：
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作者：
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DOI：
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发表时间：
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期刊：
G3 (Bethesda, Md.)
影响因子：
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作者：
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