Advances in Chromatin and Chromosome Research: Perspectives from Multiple Fields.
Advances in Chromatin and Chromosome Research: Perspectives from Multiple Fields.
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DOI：
10.1016/j.molcel.2020.07.003
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发表时间：
2020-09-17
期刊：
影响因子：
16
通讯作者：
Wadduwage D
中科院分区：
文献类型：
--
作者：
Agbleke AA;Amitai A;Buenrostro JD;Chakrabarti A;Chu L;Hansen AS;Koenig KM;Labade AS;Liu S;Nozaki T;Ovchinnikov S;Seeber A;Shaban HA;Spille JH;Stephens AD;Su JH;Wadduwage D
核小体将基因组DNA包装成染色质。通过调节DNA的转录、复制、DNA修复和表观遗传修饰，染色质形成了大多数核过程的联系。此外，染色质纤维的动态组织是基因表达调控和染色体进化成个体化姐妹体的基础，这些个体化姐妹体可以在后期干净地分离到不同的子细胞。这项合作审查聚焦于对询问染色质和染色体生物学中的关键问题至关重要的技术，包括最先进的显微镜技术，物理操纵染色质的工具，测量染色质可及性的单细胞方法，神经网络计算成像和分析工具来解释染色质结构和动力学。此外，这篇评论提供了这些工具如何应用于特定的研究领域，如基因组稳定性和发育生物学，并测试概念，如染色质的相分离的观点。在这篇合作综述中，Agbleke等人，讨论新技术的发展和应用，以探测染色质和染色体生物学问题。作者研究了新的染色质概念，从染色质社区内的研究人员以及从邻近领域的观点。
Nucleosomes package genomic DNA into chromatin. By regulating DNA access for transcription, replication, DNA repair, and epigenetic modification, chromatin forms the nexus of most nuclear processes. In addition, dynamic organization of the chromatin fiber underlies both regulation of gene expression and evolution of chromosomes into individualized sister objects which can segregate cleanly to different daughter cells at anaphase. This collaborative review shines a spotlight on technologies that will be crucial to interrogate key questions in chromatin and chromosome biology including state-of-the-art microscopy techniques, tools to physically manipulate chromatin, single-cell methods to measure chromatin accessibility, computational imaging with neural networks and analytical tools to interpret chromatin structure and dynamics. In addition, this review provides perspectives on how these tools can be applied to specific research fields such as genome stability and developmental biology, and to test concepts such as phase separation of chromatin. In this collaborative review, Agbleke et al., discuss the development and application of new technologies to probe chromatin and chromosome biology questions. The authors examine new chromatin concepts, drawing on perspectives from researchers within the chromatin community as well as from those in adjacent fields.
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影响因子：
13.6
作者：
Barth, R.;Bystricky, K.;Shaban, H. A.
通讯作者：
Shaban, H. A.
影响因子：
3.4
作者：
Bauer, Maximilian;Metzler, Ralf
通讯作者：
Metzler, Ralf
影响因子：
64.5
作者：
Banani SF;Rice AM;Peeples WB;Lin Y;Jain S;Parker R;Rosen MK
通讯作者：
Rosen MK
DOI：
10.1073/pnas.022638399
发表时间：
2002-02-19
影响因子：
11.1
作者：
Brower-Toland, BD;Smith, CL;Wang, MD
通讯作者：
Wang, MD
影响因子：
3.4
作者：
Banigan, Edward J.;Stephens, Andrew D.;Marko, John F.
通讯作者：
Marko, John F.