Probiotic Bifidobacterium bifidum G9-1 Has a Preventive Effect on the Acceleration of Colonic Permeability and M1 Macrophage Population in Maternally Separated Rats.
Probiotic Bifidobacterium bifidum G9-1 Has a Preventive Effect on the Acceleration of Colonic Permeability and M1 Macrophage Population in Maternally Separated Rats.
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DOI：
10.3390/biomedicines9060641
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发表时间：
2021-06-03
期刊：
影响因子：
4.7
通讯作者：
Miwa H
中科院分区：
文献类型：
--
作者：
Wang X;Fukui H;Ran Y;Xu X;Ebisutani N;Nakanishi T;Tanaka Y;Maeda A;Makizaki Y;Tomita T;Oshima T;Miwa H
虽然益生菌可能对治疗肠易激综合征（IBS）有用，但益生菌如何在结肠粘膜完整性和免疫力中发挥作用尚不清楚。在此，我们的目的是研究两歧双歧杆菌G9-1（BBG 9 -1）对结肠粘膜完整性和巨噬细胞行为的影响，在母体分离（MS）作为IBS模型的大鼠。将MS幼仔与其母鼠单独分开，并对部分MS大鼠经口给予BBG 9 -1。采用Ussing小室法检测结肠黏膜通透性。实时荧光定量RT-PCR和免疫组化检测结肠组织中紧密连接蛋白和细胞因子的表达以及CD 80阳性细胞的数量。用细胞因子刺激Caco 2细胞并测量跨上皮电阻（TEER）。相对于对照组，MS大鼠显示出显著更高的结肠通透性和结肠上皮中更低的claudin 4表达。MS大鼠结肠黏膜中CD 80阳性巨噬细胞数量明显增多，同时伴有IL-6和IFN-γ表达的增加。BBG 9 -1治疗可减轻MS大鼠结肠组织中M1巨噬细胞的增加和IL-6/IFN-γ的表达。同时，BBG 9 -1治疗改善了MS大鼠结肠中增强的粘膜通透性和降低的claudin 4表达。IL-6和IFN-γ在MS大鼠结肠中的表达增强，在体外显著降低Caco 2细胞的TEER。益生菌BBG 9 -1对母离大鼠结肠通透性和M1巨噬细胞群的加速具有预防作用。
Although probiotics may be useful for the treatment of irritable bowel syndrome (IBS), it is unclear how probiotics play a role in colonic mucosal integrity and immunity. Here, we aimed to investigate the effect of Bifidobacterium bifidum G9-1 (BBG9-1) on colonic mucosal integrity and macrophage behavior in rats subjected to maternal separation (MS) as a model of IBS. MS pups were individually separated from their mother rats, and a proportion of the MS rats were orally administered BBG9-1. The colonic mucosal permeability was evaluated by Ussing chamber assay. The expression of tight junction proteins and cytokines and the population of CD80-positive cells was examined in the colonic tissues by real-time reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. Caco2 cells were stimulated with cytokines and the transepithelial electric resistance (TEER) was measured. MS rats showed significantly higher colonic permeability and lower claudin 4 expression in the colonic epithelium relative to controls. The number of CD80-positive macrophages was significantly increased in the colonic mucosa of MS rats, accompanied by the increase of IL-6 and IFN-γ expression. BBG9-1 treatment ameliorated the increase of M1 macrophage and IL-6/IFN-γ expression in the colonic tissue of MS rats. Simultaneously, BBG9-1 treatment improved the enhanced mucosal permeability and the decreased claudin 4 expression in the colon of MS rats. IL-6 and IFN-γ, whose expression is enhanced in the colon of MS rats, significantly decreased TEER in Caco2 cells in vitro. Probiotic BBG9-1 has a preventive effect on the acceleration of colonic permeability and M1 macrophage population in maternally separated rats.
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影响因子：
8.8
作者：
Fukui, H.;Zhang, X.;Sun, C.;Hara, K.;Kikuchi, S.;Yamasaki, T.;Kondo, T.;Tomita, T.;Oshima, T.;Watari, J.;Imura, J.;Fujimori, T.;Sasako, M.;Miwa, H.
通讯作者：
Miwa, H.
影响因子：
4.6
作者：
Fukui H;Oshima T;Tanaka Y;Oikawa Y;Makizaki Y;Ohno H;Tomita T;Watari J;Miwa H
通讯作者：
Miwa H
影响因子：
7.8
作者：
Kitayama, Yoshitaka;Fukui, Hirokazu;Miwa, Hiroto
通讯作者：
Miwa, Hiroto
影响因子：
3.9
作者：
Fukui, Hirokazu;Nishida, Akifumi;Miwa, Hiroto
通讯作者：
Miwa, Hiroto
影响因子：
2.6
作者：
Makizaki, Yutaka;Maeda, Ayako;Yamamura, Hideki
通讯作者：
Yamamura, Hideki