Therapeutic Effects of Intra-Articular Ultrapurified Low Endotoxin Alginate Administration on Experimental Osteoarthritis in Rabbits
Therapeutic Effects of Intra-Articular Ultrapurified Low Endotoxin Alginate Administration on Experimental Osteoarthritis in Rabbits
复制标题
关节内注射超纯化低内毒素海藻酸盐对家兔实验性骨关节炎的治疗作用
作者：
T. Igarashi;N. Iwasaki;D. Kawamura;Yukinori Tsukuda;Y. Kasahara;M. Todoh;S. Tadano;A. Minami
目的：研制一种能显著降低内毒素水平的超纯低内毒素海藻酸盐。我们的目的是检验海藻酸盐对骨关节炎(OA)进展的影响，并确定海藻酸盐的适当分子量以达到治疗效果。设计：35只日本大耳白兔行前交叉韧带横断术，建立膝关节骨关节炎模型。术后4周开始关节腔内注射，每种材料0.3mL溶液，每周注射5次。将70个膝关节分为AL430(430 KDa)、AL1000(1000 KDa)、AL1700(1700 KDa)、透明质酸(HA)和生理盐水组(NS)。术后9周，对所有膝关节进行大体、组织学和力学评估。结果：与生理盐水组和透明质酸盐组相比，海藻酸组大体表现为软骨降解较轻。海藻酸组的组织学结果显示，骨性关节炎的严重程度明显减轻。Kikuchi等人的组织学评分。藻酸盐治疗组优于生理盐水组。AL1000组的摩擦系数明显低于生理盐水组和HA组。结论：本研究表明，我们合成的海藻酸盐，尤其是AL1000，在预防骨性关节炎进展方面具有潜在的应用前景。
Objective: We have developed an ultrapurified low endotoxin alginate (UPLE alginate), which can drastically reduce endotoxin levels. Our purposes were to examine the effects of UPLE alginate administration on osteoarthritis (OA) progression and to determine the adequate molecular weight of the UPLE alginate for therapeutic effects. Design: To induce knee OA, 35 Japanese White rabbits underwent anterior cruciate ligament transection. Intra-articular injections of 0.3 mL solution of each material were started at 4 weeks postoperatively for a total of 5 weekly injections. Seventy knees were divided into the following groups: AL430 (430 kDa molecular weight UPLE alginate), AL1000 (1,000 kDa), AL1700 (1,700 kDa), HA (hyaluronan), and NS (normal saline). At 9 weeks postoperatively, all knees were assessed macroscopically, histologically, and mechanically. Results: Macroscopically, the UPLE alginate groups exhibited milder cartilage degradation compared to that of the NS and HA groups. Histological findings of the UPLE alginate groups showed an obvious reduction in the severity of OA. The histological scores of Kikuchi et al. were superior in the alginate treatment groups compared to the NS group. The friction coefficient of the AL1000 group was significantly lower than that of the NS and HA groups. Conclusion: This study indicates that our UPLE alginates, especially AL1000, have promising potential as an effective agent in preventing OA progression.
影响因子：
2.9
作者：
GUO, JF;JOURDIAN, GW;MACCALLUM, DK
通讯作者：
MACCALLUM, DK
影响因子：
7
作者：
Amiel, D;Toyoguchi, T;Healey, RM
通讯作者：
Healey, RM
影响因子：
7
作者：
Yoshioka, M;Shimizu, C;Amiel, D
通讯作者：
Amiel, D