Multiple Laser Doppler Flowmetry Probes Increase the Reproducibility of Skin Blood Flow Measurements.
Multiple Laser Doppler Flowmetry Probes Increase the Reproducibility of Skin Blood Flow Measurements.
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DOI：
10.3389/fphys.2022.876633
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发表时间：
2022
影响因子：
4
通讯作者：
Cui, Jian
中科院分区：
文献类型：
--
作者：
Luck, J. Carter;Kunselman, Allen R.;Herr, Michael D.;Blaha, Cheryl A.;Sinoway, Lawrence I.;Cui, Jian
皮肤微循环灌注通常使用激光多普勒血流仪（LDF）探头进行测量，该探头提供皮肤血流（SkBF）的连续、非侵入性定量。然而，皮肤微血管密度的不均匀性会导致LDF探头被移除和更换时再现性的降低，如在干预前后或日间测量期间的情况。因此，本研究旨在确定增加局部区域中单个LDF探针的总数是否会提高测量的重现性。七个激光多普勒探头被固定在定制的丙烯酸保持器中，该保持器被设计成容易地附着到皮肤表面。在11名参与者（6名男性，5名女性，42 ± 15岁）中评估了SkBF、局部皮肤温度（Tsk）和血压（BP）。从前臂背侧（手臂试验）测量SkBF和Tsk 5分钟。接下来，将多激光装置移动到小腿的外侧（腿部试验），并获得5分钟的测量。每个手臂和腿部试验循环重复三次，所有试验间隔10-15分钟。所有可能的LDF探头组合的平均SkBF和皮肤血管传导性（CVC）在三项手臂和腿部试验中无统计学差异。使用具有绝对一致性的双向混合效应模型计算CVC的组内相关系数（ICC），最小ICC随着LDF探针的加入而增加。7个LDF穿刺针的平均CVC的ICC在手臂试验之间为0.96，在腿部试验之间为0.91，这表明在取出和更换器械后试验之间具有极好的可靠性和很小的差异。此外，来自≥3个LDF探针组合的所有单个ICC值均大于0.70（即，良好的可靠性）。这些数据表明，在同一参与者体内更换探头后，在定制的保持器中使用多个激光多普勒探头进行的SkBF测量具有良好的再现性。因此，该应用可以在重复测量之间提供更可重复的评估（例如，运动或临床手术之前和之后），其中必须在同一位置移除并更换LDF探头。
Cutaneous microcirculatory perfusion is commonly measured using laser Doppler flowmetry (LDF) probes, which provide a continuous, non-invasive quantification of skin blood flow (SkBF). However, inhomogeneities in the skin’s microvasculature density contribute to a decrease in reproducibility whenever an LDF probe is removed and replaced, as is the case during pre- and post-intervention or between-day measurements. Therefore, this study aimed to determine whether increasing the total number of individual LDF probes in a localized area improves the reproducibility of the measurement. Seven laser Doppler probes were secured in a custom-made acrylic holder designed to attach to the skin’s surface easily. SkBF, local skin temperature (Tsk), and blood pressure (BP) were assessed in 11 participants (6 M, 5 F, 42 ± 15 years). SkBF and Tsk were measured from the dorsal forearm (arm trial) for 5 min. Next, the multi-laser device was moved to the lateral side of the calf (leg trial), and measurements were obtained for 5 min. Each arm and leg trial was cyclically repeated three times, and all trials were separated by intermissions lasting 10–15 min. The average SkBF and the cutaneous vascular conductance (CVC) from all possible LDF probe combinations were not statistically different across the three arm and leg trials. Two-way mixed-effects models with absolute agreement were used to compute the intraclass correlation coefficient (ICC) for CVC, and the minimum ICC increased with the addition of LDF probes. The ICC of the average CVC from seven LDF probes was 0.96 between the arm trials and 0.91 between the leg trials, which suggests that there is excellent reliability and little difference between trials following the removal and replacement of the device. Moreover, all individual ICC values from ≥3 LDF probe combinations were greater than 0.70 (i.e., good reliability). These data suggest that SkBF measurements with multiple laser Doppler probes in a custom-made holder have excellent reproducibility after replacing the probes within the same participant. Therefore, this application could provide more reproducible assessments between repeated measurements (e.g., before and after exercise or clinical procedures) where the LDF probes must be removed and replaced within the same location.
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影响因子：
2.5
作者：
Hanne, Nicholas J.;Easter, Elizabeth D.;Cole, Jacqueline H.
通讯作者：
Cole, Jacqueline H.
影响因子：
2
作者：
Carrasco, Josep L.;Caceres, Alejandro;Jover, Lluis
通讯作者：
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影响因子：
6.5
作者：
HOLLOWAY, GA;WATKINS, DW
通讯作者：
WATKINS, DW
影响因子：
4.6
作者：
NILSSON, GE;TENLAND, T;OBERG, PA
通讯作者：
OBERG, PA
DOI：
10.1161/circheartfailure.112.000135
发表时间：
2013-03
期刊：
Circulation. Heart failure
影响因子：
--
作者：
Cui J;Boehmer JP;Blaha C;Lucking R;Kunselman AR;Sinoway LI
通讯作者：
Sinoway LI