Methylsulfonylmethane Increases the Alveolar Bone Density of Mandibles in Aging Female Mice.
Methylsulfonylmethane Increases the Alveolar Bone Density of Mandibles in Aging Female Mice.
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DOI：
10.3389/fphys.2021.708905
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发表时间：
2021
影响因子：
4
通讯作者：
Chellaiah MA
中科院分区：
文献类型：
--
作者：
Aljohani H;Senbanjo LT;Al Qranei M;Stains JP;Chellaiah MA
甲基磺酰甲烷（MSM）是一种天然存在的抗炎化合物，可有效治疗多种退行性疾病，如骨关节炎和急性胰腺炎。我们以前的研究已经证明了MSM有能力将人类脱落乳牙（SHED）的干细胞分化为成骨细胞样细胞。本研究通过在36周龄的老龄C57 BL/6雌性小鼠体内注射MSM 13周来检查MSM的全身效应。血清分析显示，在MSM注射动物中，骨形成标志物[骨钙素（OCN）和1型前胶原完整N端前肽（P1 NP）]的表达水平增加，骨吸收标志物[抗酒石酸酸性磷酸酶（TRAP）和I型胶原C端肽（CTX-I）]减少。微型计算机断层扫描图像显示下颌骨小梁密度增加。股骨中的骨小梁密度往往较高，尽管MSM和磷酸盐缓冲盐水（PBS）注射小鼠之间的增加没有显著差异。在注射MSM的小鼠中，观察到下颌骨密度增加，骨髓腔相应减少。此外，成骨细胞特异性标记物- OCN和间充质干细胞特异性标记物-CD 105的下颌骨的免疫组织化学分析分别显示OCN和CD 105阳性细胞的显著增加和减少。在对照小鼠的下颌骨的根间区域中观察到骨丢失区域。然而，由于MSM注射刺激骨形成，这种损失大大减少。总之，我们的研究已经证明了MSM在体内诱导成骨细胞形成和功能的能力，导致下颌骨骨形成增加。因此，MSM和感兴趣的干细胞的应用可能是牙周病或其他相关疾病下牙槽骨再生的正确组合。
Methylsulfonylmethane (MSM) is a naturally occurring anti-inflammatory compound that effectively treats multiple degenerative diseases such as osteoarthritis and acute pancreatitis. Our previous studies have demonstrated the ability of MSM to differentiate stem cells from human exfoliated deciduous (SHED) teeth into osteoblast-like cells. This study examined the systemic effect of MSM in 36-week-old aging C57BL/6 female mice in vivo by injecting MSM for 13 weeks. Serum analyses showed an increase in expression levels of bone formation markers [osteocalcin (OCN) and procollagen type 1 intact N-terminal propeptide (P1NP)] and a reduction in bone resorption markers [tartrate-resistant acid phosphatase (TRAP) and C-terminal telopeptide of type I collag (CTX-I)] in MSM-injected animals. Micro-computed tomographic images demonstrated an increase in trabecular bone density in mandibles. The trabecular bone density tended to be higher in the femur, although the increase was not significantly different between the MSM- and phosphate-buffered saline (PBS)-injected mice. In mandibles, an increase in bone density with a corresponding decrease in the marrow cavity was observed in the MSM-injected mice. Furthermore, immunohistochemical analyses of the mandibles for the osteoblast-specific marker – OCN, and the mesenchymal stem cell-specific marker – CD105 showed a significant increase and decrease in OCN and CD105 positive cells, respectively. Areas of bone loss were observed in the inter-radicular region of mandibles in control mice. However, this loss was considerably decreased due to stimulation of bone formation in response to MSM injection. In conclusion, our study has demonstrated the ability of MSM to induce osteoblast formation and function in vivo, resulting in increased bone formation in the mandible. Hence, the application of MSM and stem cells of interest may be the right combination in alveolar bone regeneration under periodontal or other related diseases that demonstrate bone loss.
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DOI：
10.1002/ar.20619
发表时间：
2008-01-01
影响因子：
2
作者：
Huja, Sarandeep S.;Beck, Frank M.
通讯作者：
Beck, Frank M.
DOI：
10.1083/jcb.148.4.665
发表时间：
2000-02-21
期刊：
The Journal of cell biology
影响因子：
--
作者：
Chellaiah M;Kizer N;Silva M;Alvarez U;Kwiatkowski D;Hruska KA
通讯作者：
Hruska KA
影响因子：
3.3
作者：
Chellaiah, MA;Kizer, N;Hruska, KA
通讯作者：
Hruska, KA
影响因子：
4.6
作者：
AlQranei, Mohammed S.;Aljohani, Hanan;Chellaiah, Meenakshi A.
通讯作者：
Chellaiah, Meenakshi A.
影响因子：
5.8
作者：
Chavassieux, Pascale;Portero-Muzy, Nathalie;Chapurlat, Roland
通讯作者：
Chapurlat, Roland