Rapid and complete paraffin removal from human tissue sections delivers enhanced Raman spectroscopic and histopathological analysis.
Rapid and complete paraffin removal from human tissue sections delivers enhanced Raman spectroscopic and histopathological analysis.
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DOI：
10.1039/c9an01030k
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发表时间：
2020-02-17
期刊：
影响因子：
--
通讯作者：
Thomas GMH
中科院分区：
文献类型：
--
作者：
Gaifulina R ;Caruana DJ ;Oukrif D ;Guppy NJ ;Culley S ;Brown R ;Bell I ;Rodriguez-Justo M ;Lau K ;Thomas GMH
一个简单的方法完全去除污染石蜡从样品的临床拉曼和增强免疫组织学分析。从用于诊断组织学的处理的组织中不完全去除石蜡和有机污染物一直是将拉曼光谱技术引入临床实践的深刻障碍。我们报告了一种使用超级镜面不锈钢载玻片从一系列福尔马林固定的石蜡包埋组织中快速完全去除石蜡的方法。该方法对一系列人类和动物组织同样有效，对存档和新样本同样有效，并与标准病理学实验室程序兼容。我们描述了一个一般的增强的拉曼散射和增强染色的抗体用于免疫组织化学的临床诊断。我们的结论是，这些新的载玻片基板有能力提高诊断，通过解剖病理学，促进同时结合改进，更灵敏的免疫组织化学染色和简化，更可靠的拉曼光谱成像，分析和信号处理。
A simple method completely removes contaminating paraffin from samples for clinical Raman and enhanced immunohistological analysis. Incomplete removal of paraffin and organic contaminants from tissues processed for diagnostic histology has been a profound barrier to the introduction of Raman spectroscopic techniques into clinical practice. We report a route to rapid and complete paraffin removal from a range of formalin-fixed paraffin embedded tissues using super mirror stainless steel slides. The method is equally effective on a range of human and animal tissues, performs equally well with archived and new samples and is compatible with standard pathology lab procedures. We describe a general enhancement of the Raman scatter and enhanced staining with antibodies used in immunohistochemistry for clinical diagnosis. We conclude that these novel slide substrates have the power to improve diagnosis through anatomical pathology by facilitating the simultaneous combination of improved, more sensitive immunohistochemical staining and simplified, more reliable Raman spectroscopic imaging, analysis and signal processing.
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影响因子：
4.6
作者：
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