Synthesis of poly(N-isopropylacrylamide) particles for metal affinity binding of peptides.
Synthesis of poly(N-isopropylacrylamide) particles for metal affinity binding of peptides.
复制标题
肽的金属亲和力结合的聚（N-异丙基丙烯酰胺）颗粒的合成。
DOI：
10.1016/j.colsurfb.2013.09.060
复制
发表时间：
2014-02-01
影响因子：
5.8
通讯作者：
Yates, Matthew Z.
中科院分区：
文献类型：
--
作者：
Tsai, Hsin-Yi;Lee, Alexander;Peng, Wei;Yates, Matthew Z.
制备了具有金属亲和配体的温度敏感型聚N-异丙基丙烯酰胺(PNIPAM)微凝胶颗粒，用于选择性结合含有组氨酸标签的多肽(连续6个组氨酸残基)。以聚N-乙烯基吡咯烷酮(PVP)为空间稳定剂，通过两步分散聚合，使PNIPAM粒子与功能配体VBIDA共聚。由于化学接枝了PVP链，得到的粒子尺寸单一，在广泛的温度和离子强度范围内胶体稳定。粒子大小对水环境的离子强度和pH也很敏感，可能是由于电离的VBIDA基团之间的静电斥力。二价镍离子被螯合到VBIDA基团，允许选择性金属亲和连接His6-Cys多肽。当竞争配体咪唑加入后，多肽被释放，这表明多肽与颗粒的结合是可逆的和选择性的。
Temperature-sensitive poly(N-isopropylacrylamide) (PNIPAM) microgel particles with metal affinity ligands were prepared for selective binding of peptides containing the His6-tag (six consecutive histidine residues). The PNIPAM particles were copolymerized with the functional ligand vinylbenzyl iminodiacetic acid (VBIDA) through a two-stage dispersion polymerization using poly(N-vinyl pyrrolidone) (PVP) as a steric stabilizer. The resulting particles were monodisperse in size and colloidally stable over a wide range of temperature and ionic strength due to chemically grafted PVP chains. The particle size was also found to be sensitive to ionic strength and pH of the aqueous environment, likely due to the electrostatic repulsion between ionized VBIDA groups. Divalent nickel ions were chelated to the VBIDA groups, allowing selective metal affinity attachment of a His6-Cys peptide. The peptide was released upon the addition of the competitive ligand imidazole, demonstrating that the peptide attachment to the particles is reversible and selective.
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DOI：
10.1021/la1039128
发表时间：
2010-12-07
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
--
作者：
Lee A;Tsai HY;Yates MZ
通讯作者：
Yates MZ
DOI：
10.1023/a:1008055211667
发表时间：
1998-01-01
期刊：
BIOSEPARATION
影响因子：
--
作者：
Kawaguchi, H;Fujimoto, K
通讯作者：
Fujimoto, K
影响因子：
4.4
作者：
HIROKAWA, Y;TANAKA, T
通讯作者：
TANAKA, T
影响因子：
5.8
作者：
Lopez, VC;Hadgraft, J;Snowden, MJ
通讯作者：
Snowden, MJ
影响因子：
3
作者：
ANDERSON, CC;RODRIGUEZ, F;THURSTON, DA
通讯作者：
THURSTON, DA