Oocyte aging-induced Neuronatin (NNAT) hypermethylation affects oocyte quality by impairing glucose transport in porcine.
Oocyte aging-induced Neuronatin (NNAT) hypermethylation affects oocyte quality by impairing glucose transport in porcine.
复制标题
卵母细胞老化诱导的神经元 (NNAT) 高甲基化通过损害猪体内的葡萄糖转运来影响卵母细胞质量。
DOI：
10.1038/srep36008
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发表时间：
2016-10-26
影响因子：
4.6
通讯作者：
Miao YL
中科院分区：
文献类型：
--
作者：
Gao YY;Chen L;Wang T;Nie ZW;Zhang X;Miao YL
DNA甲基化在调节许多生理行为中起着重要作用；然而，很少有研究关注卵母细胞衰老过程中DNA甲基化的变化。早期的研究表明，一些印迹基因的DNA甲基化在老年小鼠卵母细胞中发生了变化。在本研究中，我们利用猪卵母细胞来验证卵母细胞衰老会改变基因的DNA甲基化模式并干扰其在衰老卵母细胞中的表达，从而影响卵母细胞的发育潜力。我们比较了几种不同类型的基因，发现衰老卵母细胞中神经元蛋白（Neuronatin， NNAT）的表达和DNA甲基化明显受到干扰。另外的实验表明，葡萄糖转运在衰老的卵母细胞中受到损害，在新鲜的卵母细胞中注射NNAT抗体对葡萄糖转运也有同样的影响。这些结果表明，NNAT的表达通过提高DNA甲基化而降低，从而通过降低衰老卵母细胞的葡萄糖转运能力而影响卵母细胞的质量。
DNA methylation plays important roles in regulating many physiological behaviors; however, few studies were focused on the changes of DNA methylation during oocyte aging. Early studies showed that some imprinted genes’ DNA methylation had been changed in aged mouse oocytes. In this study, we used porcine oocytes to test the hypothesis that oocyte aging would alter DNA methylation pattern of genes and disturb their expression in age oocytes, which affected the developmental potential of oocytes. We compared several different types of genes and found that the expression and DNA methylation of Neuronatin (NNAT) were disturbed in aged oocytes significantly. Additional experiments demonstrated that glucose transport was impaired in aged oocytes and injection of NNAT antibody into fresh oocytes led to the same effects on glucose transport. These results suggest that the expression of NNAT was declined by elevating DNA methylation, which affected oocyte quality by decreasing the ability of glucose transport in aged oocytes.
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影响因子：
4.8
作者：
Imamura, T;Kerjean, A;Pàldi, A
通讯作者：
Pàldi, A
影响因子：
2.7
作者：
Tgarashi, Hideki;Knott, Jason G.;Williams, Carnien J.
通讯作者：
Williams, Carnien J.
影响因子：
4.8
作者：
Joe, Myung Kuk;Lee, Hyo Jung;Jung, Myeong Ho
通讯作者：
Jung, Myeong Ho
影响因子：
8
作者：
Gu, Ling;Liu, Honglin;Gu, Xi;Boots, Christina;Moley, Kelle H.;Wang, Qiang
通讯作者：
Wang, Qiang
小鼠卵丘细胞中的葡萄糖代谢通过维持能量供应和细胞内氧化还原电位来防止卵母细胞老化
影响因子：
3.6
作者：
Li, Qing;Miao, De-Qiang;Tan, Jing-He
通讯作者：
Tan, Jing-He