Single-molecule fluorescence measurements of ribosomal translocation dynamics.
Single-molecule fluorescence measurements of ribosomal translocation dynamics.
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DOI：
10.1016/j.molcel.2011.03.024
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发表时间：
2011-05-06
期刊：
影响因子：
16
通讯作者：
Cooperman BS
中科院分区：
文献类型：
--
作者：
Chen C;Stevens B;Kaur J;Cabral D;Liu H;Wang Y;Zhang H;Rosenblum G;Smilansky Z;Goldman YE;Cooperman BS
We employ single-molecule fluorescence resonance energy transfer (smFRET) to study structural dynamics over the first two elongation cycles of protein synthesis, using ribosomes containing either Cy3-labeled ribosomal protein L11 and A- or P-site Cy5-labeled tRNA or Cy3 and Cy5 labeled tRNAs. Pre-translocation (PRE) complexes demonstrate fluctuations between classical and hybrid forms, with concerted motions of tRNAs away from L11 and from each other when classical complex converts to hybrid complex. EF-G·GTP binding to both hybrid and classical PRE complexes halts these fluctuations prior to catalyzing translocation to form the post-translocation (POST) complex. EF-G dependent translocation from the classical PRE complex proceeds via transient formation of a short-lived hybrid intermediate. A-site binding of either EF-G to the PRE complex or of aminoacyl-tRNA·EF-Tu ternary complex to the POST complex markedly suppresses ribosome conformational lability.
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影响因子：
5.6
作者：
Grigoriadou, Christina;Marzi, Stefano;Cooperman, Barry S.
通讯作者：
Cooperman, Barry S.
影响因子：
6.1
作者：
Agirrezabala, Xabier;Frank, Joachim
通讯作者：
Frank, Joachim
影响因子：
16
作者：
Marshall, R. Andrew;Aitken, Colin Echeverria;Puglisi, Joseph D.
通讯作者：
Puglisi, Joseph D.
DOI：
10.1073/pnas.0805299105
发表时间：
2008-10-07
影响因子：
11.1
作者：
Marshall, R. Andrew;Dorywalska, Magdalena;Puglisi, Joseph D.
通讯作者：
Puglisi, Joseph D.
影响因子：
16.6
作者：
Frank J;Gonzalez RL Jr
通讯作者：
Gonzalez RL Jr