Regulation of cytoplasmic dynein function in vivo by the Drosophila Glued complex.
Regulation of cytoplasmic dynein function in vivo by the Drosophila Glued complex.
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DOI：
10.1083/jcb.131.2.411
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发表时间：
1995-10
影响因子：
7.8
通讯作者：
HAYS, TS
中科院分区：
文献类型：
--
作者：
MCGRAIL, M;GEPNER, J;SILVANOVICH, A;LUDMANN, S;SERR, M;HAYS, TS
果蝇Glued基因产物与脊椎动物dynactin的p150组分具有序列同源性。Dynactin是一种多蛋白复合物，在体外刺激细胞质动力蛋白介导的囊泡运动。在这份报告中，我们提出了生物化学，细胞学和遗传学的证据，表明果蝇胶合复合物和脊椎动物的动力蛋白之间的功能相似性。我们表明，类似于脊椎动物的同源物中的dynactin，胶合多肽的20 S复合物的组成部分。我们的生物化学研究进一步揭示了Glued多肽的差异表达，所有这些多肽都是微管相关蛋白。在我们的分析中，由显性突变，Glued编码的Glued多肽，我们确定了一个截短的多肽，未能组装成野生型20 S复合物，但保留了与微管共纯化的能力。在卵子发生过程中的胶复合物的空间和时间分布所示的免疫细胞化学方法是相同的细胞质动力蛋白先前所描述的模式。值得注意的是，卵子发生中Glued分布的模式依赖于动力蛋白功能，以及已知正确的动力蛋白定位所需的其他几种基因产物。在遗传互补研究中，我们发现细胞质动力蛋白重链基因Dhc64C中的某些突变作为显性Glued突变的粗糙眼表型的显性抑制因子或增强因子。此外，我们发现，以前分离的Glued突变的抑制基因突变是Dhc64C的等位基因。与所观察到的依赖性的Glued本地化动力蛋白功能，这些遗传相互作用表明果蝇动力蛋白电机和Glued复合物之间的功能关联。
The Drosophila Glued gene product shares sequence homology with the p150 component of vertebrate dynactin. Dynactin is a multiprotein complex that stimulates cytoplasmic dynein-mediated vesicle motility in vitro. In this report, we present biochemical, cytological, and genetic evidence that demonstrates a functional similarity between the Drosophila Glued complex and vertebrate dynactin. We show that, similar to the vertebrate homologues in dynactin, the Glued polypeptides are components of a 20S complex. Our biochemical studies further reveal differential expression of the Glued polypeptides, all of which copurify as microtubule-associated proteins. In our analysis of the Glued polypeptides encoded by the dominant mutation, Glued, we identify a truncated polypeptide that fails to assemble into the wild-type 20S complex, but retains the ability to copurify with microtubules. The spatial and temporal distribution of the Glued complex during oogenesis is shown by immunocytochemistry methods to be identical to the pattern previously described for cytoplasmic dynein. Significantly, the pattern of Glued distribution in oogenesis is dependent on dynein function, as well as several other gene products known to be required for proper dynein localization. In genetic complementation studies, we find that certain mutations in the cytoplasmic dynein heavy chain gene Dhc64C act as dominant suppressors or enhancers of the rough eye phenotype of the dominant Glued mutation. Furthermore, we show that a mutation that was previously isolated as a suppressor of the Glued mutation is an allele of Dhc64C. Together with the observed dependency of Glued localization on dynein function, these genetic interactions demonstrate a functional association between the Drosophila dynein motor and Glued complexes.
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DOI：
10.1083/jcb.118.6.1333
发表时间：
1992-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Corthésy-Theulaz I;Pauloin A;Pfeffer SR
通讯作者：
Pfeffer SR
DOI：
10.1083/jcb.127.1.129
发表时间：
1994-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Clark SW;Meyer DI
通讯作者：
Meyer DI
影响因子：
7.8
作者：
Gardner, L C;O'Toole, E;Perrone, C A;Giddings, T;Porter, M E
通讯作者：
Porter, M E
DOI：
10.1083/jcb.126.6.1475
发表时间：
1994-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Li M;McGrail M;Serr M;Hays TS
通讯作者：
Hays TS
影响因子：
64.8
作者：
HOLZBAUR, ELF;HAMMARBACK, JA;VALLEE, RB
通讯作者：
VALLEE, RB