Identification and quantification of apo-lycopenals in fruits, vegetables, and human plasma.
Identification and quantification of apo-lycopenals in fruits, vegetables, and human plasma.
复制标题
DOI：
10.1021/jf100415z
复制
发表时间：
2010-03-24
影响因子：
6.1
通讯作者：
Schwartz SJ
中科院分区：
文献类型：
--
作者：
Kopec RE;Riedl KM;Harrison EH;Curley RW Jr;Hruszkewycz DP;Clinton SK;Schwartz SJ
研究表明，番茄红素可能通过β-胡萝卜素加氧酶2（BCO 2）催化的偏心裂解代谢，导致脱番茄红素的产生。以前曾报道过生番茄中含有载脂蛋白-6 ′-甘草醛和载脂蛋白-8 ′-甘草醛。我们现在发现，其他几种脱辅基溶血素也存在于生食和加工食品以及人体血浆中。采用番茄红素体外氧化制备脱辅基番茄红素醛标准品，建立了液相色谱-串联质谱（HPLC-MS/MS）负离子模式分离检测脱辅基番茄红素醛6′-、8′-、10′-、12′-、14′-和15′-产物的方法。分析了生番茄、红葡萄柚、西瓜和加工番茄制品的己烷/丙酮提取物，以及食用番茄汁8周的个体的血浆。在所有供试食品以及血浆中检测并定量了Apo-6′-、8′-、10′-、12′-和14′-溶血素。脱辅基物质的总和为6.5 µg/100 g“罗马”番茄、73.4 µg/100 g番茄酱和1.9 nmol/L血浆。我们的结论是，除了apo-6′-和8′-番茄红素醛外，几种apo-番茄红素醛也存在于含番茄红素的食品中。此外，血浆中存在脱辅基甘草酸可能是由于直接从食物和/或人体代谢中吸收脱辅基甘草酸所致。
Research has suggested that lycopene may be metabolized by eccentric cleavage, catalyzed by β-carotene oxygenase 2 (BCO2), resulting in the generation of apo-lycopenals. Apo-6′-lycopenal and apo-8′-lycopenal have been reported previously in raw tomato. We now show that several other apo-lycopenals are also present in raw and processed foods, as well as in human plasma. Apo-lycopenal standards were prepared by in vitro oxidation of lycopene, and a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method using atmospheric pressure chemical ionization in negative mode was developed to separate and detect the apo-6′-, 8′-, 10′-, 12′-, 14′-, and 15′-lycopenal products formed in the reaction. Hexane/acetone extracts of raw tomato, red grapefruit, watermelon, and processed tomato products were analyzed, as well as plasma of individuals who had consumed tomato juice for eight weeks. Apo-6′-, 8′-, 10′-, 12′-, and 14′-lycopenals were detected and quantified in all food products tested, as well as plasma. The sum of apo-lycopenals was 6.5 µg/100 g ‘Roma’ tomato, 73.4 µg/100 g tomato paste, and 1.9 nmol/L of plasma. We conclude that several apo-lycopenals, in addition to apo-6′- and 8′-lycopenal, are present in lycopene containing foods. In addition, the presence of apo-lycopenals in plasma may derive from the absorption of apo-lycopenals directly from food and/or human metabolism.
登录
查看更多内容
影响因子：
4.8
作者：
Kiefer, C;Hessel, S;von Lintig, J
通讯作者：
von Lintig, J
影响因子：
6.1
作者：
HAKALA, SH;HEINONEN, IM
通讯作者：
HEINONEN, IM
影响因子：
4.3
作者：
Aust, O;Ale-Agha, N;Stahl, W
通讯作者：
Stahl, W
影响因子：
4.7
作者：
Lian, Fuzhi;Smith, Donald E.;Wang, Xiang-Dong
通讯作者：
Wang, Xiang-Dong
影响因子：
3.2
作者：
Heber, D;Lu, QY
通讯作者：
Lu, QY