Functional neuropathology of neonatal hypoxia-ischemia by single-mouse longitudinal electroencephalography.
Functional neuropathology of neonatal hypoxia-ischemia by single-mouse longitudinal electroencephalography.
复制标题
DOI：
10.1111/epi.17403
复制
发表时间：
2022-12
期刊：
影响因子：
5.6
通讯作者：
Galindo, Rafael
中科院分区：
文献类型：
--
作者：
Johnson, Kevin J.;Moy, Brianna;Rensing, Nicholas;Robinson, Alexia;Ly, Michael;Chengalvala, Ramya;Wong, Michael;Galindo, Rafael
新生儿脑缺氧缺血（HI）导致症状性癫痫发作和长期神经发育障碍。啮齿动物新生儿HI的Rice-Vannucci模型已被广泛用于检查和翻译急性和慢性HI诱导的脑病的功能后果。然而，这种脑损伤模型的纵向电生理表征受到新生小鼠头部大小和出生后母体依赖性的限制。我们克服了这一挑战，采用一种新的方法，纵向单小鼠脑电图（EEG）长期植入颅内电极在长期等效的小鼠幼崽。我们描述了新生儿脑损伤后急性和慢性阶段清醒和睡眠状态下发生的神经生理学紊乱。C57 BL/6小鼠在出生后第9天进行长期双侧颅内下EEG和肌电图电极放置，然后在第二天进行单侧颈动脉烧灼和暴露于40分钟的缺氧。EEG记录之前，期间，和间歇性HI程序后，从出生后第10天到断奶年龄。定量脑电图和快速傅立叶变换分析用于评估癫痫发作，皮质脑功能障碍，和警觉状态的障碍。我们观察到新生儿HI引起的电图局灶性和双侧癫痫发作期间或紧接着全球缺氧和最常见的对侧缺血性损伤。未见自发性慢性癫痫发作。受伤的小鼠在所有频率上都出现了长期不对称的EEG背景衰减，最突出的是在非快速眼动（NREM）睡眠期间。HI小鼠在损伤后的前2天内也表现出警觉状态持续时间和转换的短暂损伤。EEG记录的小鼠新生儿HI的功能负荷与受伤的人类新生儿的功能负荷非常相似。在这种不成熟的模型中使用单小鼠纵向EEG可以促进我们对新生儿脑损伤的发育和病理生理机制的理解，并有助于转化针对这种破坏性疾病的新治疗策略。
Neonatal cerebral hypoxia-ischemia (HI) results in symptomatic seizures and long-term neurodevelopmental disability. The Rice-Vannucci model of rodent neonatal HI has been used extensively to examine and translate the functional consequences of acute and chronic HI-induced encephalopathy. Yet, longitudinal electrophysiological characterization of this brain injury model has been limited by the size of the neonatal mouse's head and postnatal maternal dependency. We overcome this challenge by employing a novel method of longitudinal single-mouse electroencephalography (EEG) using chronically implanted subcranial electrodes in the term-equivalent mouse pup. We characterize the neurophysiological disturbances occurring during awake and sleep states in the acute and chronic phases following newborn brain injury. C57BL/6 mice underwent long-term bilateral subcranial EEG and electromyographic electrode placement at postnatal day 9 followed by unilateral carotid cauterization and exposure to 40 minutes of hypoxia the following day. EEG recordings were obtained prior, during, and intermittently after the HI procedure from postnatal day 10 to weaning age. Quantitative EEG and fast Fourier transform analysis were used to evaluate seizures, cortical cerebral dysfunction, and disturbances in vigilance states. We observed neonatal HI-provoked electrographic focal and bilateral seizures during or immediately following global hypoxia and most commonly contralateral to the ischemic injury. Spontaneous chronic seizures were not seen. Injured mice developed long-term asymmetric EEG background attenuation in all frequencies and most prominently during non–rapid eye movement (NREM) sleep. HI mice also showed transient impairments in vigilance state duration and transitions during the first 2 days following injury. The functional burden of mouse neonatal HI recorded by EEG resembles closely that of the injured human newborn. The use of single-mouse longitudinal EEG in this immature model can advance our understanding of the developmental and pathophysiological mechanisms of neonatal cerebral injury and help translate novel therapeutic strategies against this devastating condition.
登录
查看更多内容
影响因子：
8
作者：
Murray, Deirdre M.;Boylan, Geraldine B.;Connolly, Sean
通讯作者：
Connolly, Sean
年龄和性别依赖性对苯巴比妥的新生儿癫痫发作的敏感性：氯化物共发作用的作用。
影响因子：
5.3
作者：
Kang SK;Markowitz GJ;Kim ST;Johnston MV;Kadam SD
通讯作者：
Kadam SD
影响因子：
7.2
作者：
Mathew JL;Kaur N;Dsouza JM
通讯作者：
Dsouza JM
影响因子：
11.2
作者：
JENSEN, FE;APPLEGATE, CD;BURCHFIEL, JL
通讯作者：
BURCHFIEL, JL
DOI：
10.1097/00004647-199805000-00012
发表时间：
1998-05-01
影响因子：
6.3
作者：
Kitagawa, K;Matsumoto, M;Yanagihara, T
通讯作者：
Yanagihara, T