The Tomato yellow leaf curl virus V2 protein forms aggregates depending on the cytoskeleton integrity and binds viral genomic DNA.
The Tomato yellow leaf curl virus V2 protein forms aggregates depending on the cytoskeleton integrity and binds viral genomic DNA.
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DOI：
10.1038/srep09967
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发表时间：
2015-05-05
影响因子：
4.6
通讯作者：
Gorovits R
中科院分区：
文献类型：
--
作者：
Moshe A;Belausov E;Niehl A;Heinlein M;Czosnek H;Gorovits R
番茄黄卷叶病毒（TYLCV）在侵染番茄细胞的细胞质和细胞核中形成了越来越大的外壳蛋白（CP）聚集体。为了更好地了解tylcv -宿主相互作用，我们研究了非结构病毒蛋白V2的性质和亚细胞积累模式。CP和V2是病毒环状单链DNA基因组上仅有的两个定向基因。与CP类似，随着感染的进展，在细胞核中也发现V2定位于越来越大的细胞质聚集体，在那里它与CP共定位。V2与病毒基因组DNA分子相关，表明V2具有DNA穿梭蛋白的功能。V2聚集体的形成和26S蛋白酶体介导的降解依赖于肌动蛋白和微管细胞骨架的完整性。我们认为，细胞骨架依赖性V2聚集体的形成和生长在TYLCV感染过程中发挥了重要作用，微管和肌动蛋白丝对于V2向26S蛋白酶体的传递很重要。
The spread of Tomato yellow leaf curl virus (TYLCV) was accompanied by the formation of coat protein (CP) aggregates of increasing size in the cytoplasm and nucleus of infected tomato (Solanum lycopersicum) cells. In order to better understand the TYLCV-host interaction, we investigated the properties and the subcellular accumulation pattern of the non-structural viral protein V2. CP and V2 are the only sense-oriented genes on the virus circular single-stranded DNA genome. Similar to CP, V2 localized to cytoplasmic aggregates of increasing size and as infection progressed was also found in nuclei, where it co-localized with CP. V2 was associated with viral genomic DNA molecules, suggesting that V2 functions as a DNA shuttling protein. The formation and the 26S proteasome-mediated degradation of V2 aggregates were dependent on the integrity of the actin and microtubule cytoskeleton. We propose that the cytoskeleton-dependent formation and growth of V2 aggregates play an important role during TYLCV infection, and that microtubules and actin filaments are important for the delivery of V2 to the 26S proteasome.
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影响因子：
5.4
作者：
Bendahmane, M;Fitchen, JH;Beachy, RN
通讯作者：
Beachy, RN
影响因子：
2.7
作者：
Baluska, F;Jasik, J;Volkmann, D
通讯作者：
Volkmann, D
影响因子：
7.4
作者：
Niehl, Annette;Amari, Khalid;Heinlein, Manfred
通讯作者：
Heinlein, Manfred
DOI：
10.1073/pnas.95.19.11169
发表时间：
1998-09-15
影响因子：
11.1
作者：
Reichel, C;Beachy, RN
通讯作者：
Beachy, RN
影响因子：
3.7
作者：
Gorovits R;Moshe A;Ghanim M;Czosnek H
通讯作者：
Czosnek H