Recruitment of the host plant heat shock protein 70 by Tomato yellow leaf curl virus coat protein is required for virus infection.
Recruitment of the host plant heat shock protein 70 by Tomato yellow leaf curl virus coat protein is required for virus infection.
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DOI：
10.1371/journal.pone.0070280
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Czosnek H
中科院分区：
文献类型：
--
作者：
Gorovits R;Moshe A;Ghanim M;Czosnek H
一种功能性衣壳蛋白（CP）是番茄黄卷叶病毒（TYLCV）和其他单株begomovirus侵染宿主植物和昆虫传播所必需的。我们之前已经证明，TYLCV CP特异性地与病毒昆虫载体烟粉虱的热休克蛋白70 （HSP70）相互作用。本研究表明，在TYLCV侵染番茄植株的过程中，大量HSP70从可溶性形态转变为不溶性聚集体。CP和HSP70在这些聚集体中共同定位，首先在细胞质中，然后在与血管系统相关的细胞核中。在叶和茎的核提取物中，共免疫沉淀证实了CP-HSP70的相互作用。槲皮素抑制HSP70表达导致细胞核CP聚集量减少，GFP-CP融合蛋白从细胞核重新定位到细胞质。HSP70失活导致TYLCV DNA水平降低，证明了HSP70在植物TYLCV增殖中的作用。本研究首次揭示了植物HSP70参与TYLCV CP细胞内运动。如前所述，核聚集体含有TYLCV DNA-CP复合物和感染性病毒粒子。表明HSP70定位于这些大的核聚集体，可以推断这些结构是核病毒工厂。
A functional capsid protein (CP) is essential for host plant infection and insect transmission of Tomato yellow leaf curl virus (TYLCV) and other monopartite begomoviruses. We have previously shown that TYLCV CP specifically interacts with the heat shock protein 70 (HSP70) of the virus insect vector, Bemisia tabaci. Here we demonstrate that during the development of tomato plant infection with TYLCV, a significant amount of HSP70 shifts from a soluble form into insoluble aggregates. CP and HSP70 co-localize in these aggregates, first in the cytoplasm, then in the nucleus of cells associated with the vascular system. CP-HSP70 interaction was demonstrated by co-immunopreciptation in cytoplasmic - but not in nuclear extracts from leaf and stem. Inhibition of HSP70 expression by quercetin caused a decrease in the amount of nuclear CP aggregates and a re-localization of a GFP-CP fusion protein from the nucleus to the cytoplasm. HSP70 inactivation resulted in a decrease of TYLCV DNA levels, demonstrating the role of HSP70 in TYLCV multiplication in planta. The current study reveals for the first time the involvement of plant HSP70 in TYLCV CP intracellular movement. As described earlier, nuclear aggregates contained TYLCV DNA-CP complexes and infectious virions. Showing that HSP70 localizes in these large nuclear aggregates infers that these structures operate as nuclear virus factories.
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影响因子：
3.5
作者：
Gorovits, Rena;Akad, Fouad;Czosnek, Henryk
通讯作者：
Czosnek, Henryk
影响因子：
5.4
作者：
Arguello-Astorga, G;Lopez-Ochoa, L;Hanley-Bowdoin, L
通讯作者：
Hanley-Bowdoin, L
影响因子：
3.2
作者：
Ghanim, M;Morin, S;Czosnek, H
通讯作者：
Czosnek, H
影响因子：
3.7
作者：
DAVIES, C;HILLS, G;BAULCOMBE, DC
通讯作者：
BAULCOMBE, DC
影响因子：
3.3
作者：
Gutierrez, C;Ramirez-Parra, E;Missich, R
通讯作者：
Missich, R