A novel candidate vaccine for cytauxzoonosis inferred from comparative apicomplexan genomics.
A novel candidate vaccine for cytauxzoonosis inferred from comparative apicomplexan genomics.
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DOI：
10.1371/journal.pone.0071233
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Birkenheuer AJ
中科院分区：
文献类型：
--
作者：
Tarigo JL;Scholl EH;McK Bird D;Brown CC;Cohn LA;Dean GA;Levy MG;Doolan DL;Trieu A;Nordone SK;Felgner PL;Vigil A;Birkenheuer AJ
Cytauxzoonosis is an emerging infectious disease of domestic cats (Felis catus) caused by the apicomplexan protozoan parasite Cytauxzoon felis. The growing epidemic, with its high morbidity and mortality points to the need for a protective vaccine against cytauxzoonosis. Unfortunately, the causative agent has yet to be cultured continuously in vitro, rendering traditional vaccine development approaches beyond reach. Here we report the use of comparative genomics to computationally and experimentally interpret the C. felis genome to identify a novel candidate vaccine antigen for cytauxzoonosis. As a starting point we sequenced, assembled, and annotated the C. felis genome and the proteins it encodes. Whole genome alignment revealed considerable conserved synteny with other apicomplexans. In particular, alignments with the bovine parasite Theileria parva revealed that a C. felis gene, cf76, is syntenic to p67 (the leading vaccine candidate for bovine theileriosis), despite a lack of significant sequence similarity. Recombinant subdomains of cf76 were challenged with survivor-cat antiserum and found to be highly seroreactive. Comparison of eleven geographically diverse samples from the south-central and southeastern USA demonstrated 91–100% amino acid sequence identity across cf76, including a high level of conservation in an immunogenic 226 amino acid (24 kDa) carboxyl terminal domain. Using in situ hybridization, transcription of cf76 was documented in the schizogenous stage of parasite replication, the life stage that is believed to be the most important for development of a protective immune response. Collectively, these data point to identification of the first potential vaccine candidate antigen for cytauxzoonosis. Further, our bioinformatic approach emphasizes the use of comparative genomics as an accelerated path to developing vaccines against experimentally intractable pathogens.
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DOI：
10.1083/jcb.113.1.87
发表时间：
1991-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Shaw MK;Tilney LG;Musoke AJ
通讯作者：
Musoke AJ
影响因子：
2.6
作者：
Cohn, L. A.;Birkenheuer, A. J.;Craig, A. W.
通讯作者：
Craig, A. W.
影响因子：
9.4
作者：
Birkenheuer, AJ;Levy, MG;Breitschwerdt, EB
通讯作者：
Breitschwerdt, EB
影响因子：
2.6
作者：
Freeman, Jeanne M.;Kappmeyer, Lowell S.;Knowles, Donald P.
通讯作者：
Knowles, Donald P.
影响因子：
5.5
作者：
Musoke, A;Rowlands, J;Morzaria, S
通讯作者：
Morzaria, S