Detection of pathogens in water: from phylochips to qPCR to pyrosequencing.
Detection of pathogens in water: from phylochips to qPCR to pyrosequencing.
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DOI：
10.1016/j.copbio.2011.11.016
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发表时间：
2012-06
影响因子：
7.7
通讯作者：
Rose JB
中科院分区：
文献类型：
--
作者：
Aw TG;Rose JB
我们回顾了用于检测水中病原体的分子工具的最新进展。微阵列、qPCR和焦磷酸测序正迅速成为病原体检测的工具。常规应用分子工具进行水传播病原体检测仍然存在挑战。水生病原体对人类健康构成重大威胁，对微生物水质进行适当评估对于水基础设施和处理投资决策以及最终提供疾病预警非常重要，特别是考虑到与严重公共卫生风险相关的全球灾害日益增加。近年来，微生物水质监测经历了巨大的转变，新的分子工具开始提供快速，高通量，灵敏和特异性检测各种微生物病原体，挑战传统的基于培养的技术。高密度微阵列、定量实时PCR（qPCR）和焦磷酸测序被认为是由“分子革命”产生的突破性技术，目前正迅速成为病原体检测和发现的工具。未来的挑战在于将这些分子工具与浓缩技术和生物信息学平台相结合，以公正地指导水中的病原体监测并制定标准化方案。
► We review recent advances in molecular tools for detecting pathogens in water. ► Microarrays, qPCR and pyrosequencing are emerging rapidly as tools of pathogen detection. ► Challenges remain in applying molecular tools for waterborne pathogen detection on a routine basis. Waterborne pathogens pose a significant threat to human health and a proper assessment of microbial water quality is important for decision making regarding water infrastructure and treatment investments and eventually to provide early warning of disease, particularly given increasing global disasters associated with severe public health risks. Microbial water quality monitoring has undergone tremendous transition in recent years, with novel molecular tools beginning to offer rapid, high-throughput, sensitive and specific detection of a wide spectrum of microbial pathogens that challenge traditional culture-based techniques. High-density microarrays, quantitative real-time PCR (qPCR) and pyrosequencing which are considered to be breakthrough technologies borne out of the ‘molecular revolution’ are at present emerging rapidly as tools of pathogen detection and discovery. Future challenges lie in integrating these molecular tools with concentration techniques and bioinformatics platforms for unbiased guide of pathogen surveillance in water and developing standardized protocols.
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作者：
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