Identification of Burkholderia pseudomallei Near-Neighbor Species in the Northern Territory of Australia.
Identification of Burkholderia pseudomallei Near-Neighbor Species in the Northern Territory of Australia.
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DOI：
10.1371/journal.pntd.0003892
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发表时间：
2015
影响因子：
3.8
通讯作者：
Keim P
中科院分区：
文献类型：
--
作者：
Ginther JL;Mayo M;Warrington SD;Kaestli M;Mullins T;Wagner DM;Currie BJ;Tuanyok A;Keim P
近邻物种的识别和表征对于开发生物威胁剂的强大分子诊断工具至关重要。一种这样的病原体，类鼻疽伯克霍尔德氏菌，一种土壤细菌和类鼻疽的病原体，由于其基因组多样性和广泛的地理分布，在该地区缺乏。伯克霍尔德氏菌属包含60多个物种，并占据了广泛的环境，包括土壤，植物，根际，水，动物和人类。在新地点鉴定新种需要鉴定伯克霍尔德菌属种的真实全球分布，特别是与B密切相关的成员。假鼻疽在我们目前的研究中，我们使用伯克霍尔德氏菌特异性recA测序分析环境样本从达尔文地区在澳大利亚的北领地，其中类鼻疽是地方病。使用16 s rRNA测序进一步表征伯克霍尔德氏菌recA PCR阴性样品，用于种属鉴定。系统发育分析表明，超过70%的细菌分离物被鉴定为B。ubonensis表明该种常见于土壤中B.类鼻疽是地方病。贝叶斯系统发育分析揭示了B内的许多新分支。cepacia complex，一个新的B. oklahomensis-like物种，和一个新的分支包含一个分离物，这是不同于所有其他样品的系统发育树。在recA测序分析中，我们在B的反向启动区发现了2个单核苷酸多态性。Oklahomensis.一个简并引物的开发，并提出了未来的使用。我们的结论是recA测序技术是一种有效的工具，分类伯克霍尔德氏菌和确定类鼻疽流行区的土壤生物。 伯克霍尔德氏菌是一种分布广泛的细菌属，包含60多个物种。伯克霍尔德氏菌属中的物种范围从环境重要的，如分解污染物的分离株，到人类病原体。类鼻疽伯克霍尔德氏菌是类鼻疽病的病原体，是澳大利亚北方的地方病，是该地区的主要公共卫生问题。我们使用recA测序对152个未知的环境分离株进行了鉴定，以确定该地区是否存在伯克霍尔德菌。本研究中鉴定的大多数细菌属于B。发现ubonensis种和各种种与人类病原体B栖息在相同的环境样品中。假鼻疽B的角色。ubonensis和其他伯克霍尔德氏菌物种在这个地方性地区，以及他们如何与B相互作用。为了了解这种重要的人类病原体的进化，类鼻疽是需要解决的重要问题。虽然它们占据相似的地理范围，但它们必须存在于不同的生态位中，以保持不同的物种。
Identification and characterization of near-neighbor species are critical to the development of robust molecular diagnostic tools for biothreat agents. One such agent, Burkholderia pseudomallei, a soil bacterium and the causative agent of melioidosis, is lacking in this area because of its genomic diversity and widespread geographic distribution. The Burkholderia genus contains over 60 species and occupies a large range of environments including soil, plants, rhizospheres, water, animals and humans. The identification of novel species in new locations necessitates the need to identify the true global distribution of Burkholderia species, especially the members that are closely related to B. pseudomallei. In our current study, we used the Burkholderia-specific recA sequencing assay to analyze environmental samples from the Darwin region in the Northern Territory of Australia where melioidosis is endemic. Burkholderia recA PCR negative samples were further characterized using 16s rRNA sequencing for species identification. Phylogenetic analysis demonstrated that over 70% of the bacterial isolates were identified as B. ubonensis indicating that this species is common in the soil where B. pseudomallei is endemic. Bayesian phylogenetic analysis reveals many novel branches within the B. cepacia complex, one novel B. oklahomensis-like species, and one novel branch containing one isolate that is distinct from all other samples on the phylogenetic tree. During the analysis with recA sequencing, we discovered 2 single nucleotide polymorphisms in the reverse priming region of B. oklahomensis. A degenerate primer was developed and is proposed for future use. We conclude that the recA sequencing technique is an effective tool to classify Burkholderia and identify soil organisms in a melioidosis endemic area. Burkholderia is a widespread genus of bacteria that contains over 60 species. The species within Burkholderia range from environmentally important, such as isolates that breakdown pollutants, to human pathogens. Burkholderia pseudomallei, the causative agent of melioidosis, is endemic to Northern Australia and is a major public health concern in that region. We characterized 152 unknown environmental isolates using recA sequencing to identify the presence of Burkholderia bacteria in the region. The majority of bacteria identified in this study belonged to the B. ubonensis species and various species were found to inhabit the same environmental sample as the human pathogen, B. pseudomallei. The role of B. ubonensis and other Burkholderia species identified in this endemic region and how they interact with B. pseudomallei are important questions to address in order to understand the evolution of this important human pathogen. While they are occupying a similar geographic range, they must be exist in different niche in order to remain distinct species.
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影响因子：
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