Different thymosin Beta 4 immunoreactivity in foetal and adult gastrointestinal tract.
Different thymosin Beta 4 immunoreactivity in foetal and adult gastrointestinal tract.
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胎儿和成人胃肠道中的不同胸腺素β4免疫反应性。
DOI：
10.1371/journal.pone.0009111
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发表时间：
2010-02-09
期刊：
影响因子：
3.7
通讯作者：
Faa, Gavino
中科院分区：
文献类型：
--
作者：
Nemolato, Sonia;Cabras, Tiziana;Cau, Flaviana;Fanari, Mattia Umberto;Fanni, Daniela;Manconi, Barbara;Messana, Irene;Castagnola, Massimo;Faa, Gavino
胸腺素β4(Tβ4)是β胸腺素家族的一员，在多种细胞功能中发挥重要作用。我们小组最近的一项研究表明，Tβ4在人类唾液腺的发育中发挥了作用。本研究的目的是分析Tβ4在人发育过程中肠道和成人肠道中的表达。本文研究了T-β-4在两名胎儿和两名成人的舌、食道、胃、回肠、结肠、肝脏和胰腺中的免疫定位。T-β-4分布不均，胎儿与成人间差异显著。在胃中，胎儿浅表上皮呈阳性反应，成体呈阴性反应。回肠肠上皮细胞在成体呈强阳性，在胎儿呈弱阳性。与胎儿相比，成人浅表结肠上皮中Tβ4的反应性增强。胎肝和成人肝有明显的不同：前者对Tβ-4的反应性很低，而在成人的肝细胞中绝大多数肝细胞有很强的反应性。在胰腺中发现了一种特殊的模式，在胎儿和成人胰岛细胞中观察到了最强的反应。我们的数据显示，在发育过程中，Tβ4在人的肠道和内分泌胰腺中有强烈的表达。观察到的Tβ4的差异表达表明该肽在胎儿和成人的肠道中具有特定的作用。在评估Tβ4在人类胚胎发育中的作用时，应考虑到Tβ4在胎儿生活中表达的异质性，从非常罕见的肝细胞表达到内分泌胰腺的弥漫性反应。需要进一步的研究来阐明Tβ4和器官发生之间的联系。
Thymosin beta 4 (Tβ4) is a member of beta-thymosins, a family of peptides that play essential roles in many cellular functions. A recent study from our group suggested a role for Tβ4 in the development of human salivary glands. The aim of this study was to analyze the expression of Tβ4 in the human gut during development, and in the adult. Immunolocalization of Tβ4 was studied in autoptic samples of tongue, oesophagus, stomach, ileum, colon, liver and pancreas obtained from two human foetuses and two adults. Tβ4 appeared unevenly distributed, with marked differences between foetuses and adults. In the stomach, superficial epithelium was positive in foetuses and negative in adults. Ileal enterocytes were strongly positive in the adult and weakly positive in the foetuses. An increase in reactivity for Tβ4 was observed in superficial colon epithelium of adults as compared with the foetuses. Striking differences were found between foetal and adult liver: the former showed a very low reactivity for Tβ4 while in the adult we observed a strong reactivity in the vast majority of the hepatocytes. A peculiar pattern was found in the pancreas, with the strongest reactivity observed in foetal and adult islet cells. Our data show a strong expression of Tβ4 in the human gut and in endocrine pancreas during development. The observed differential expression of Tβ4 suggests specific roles of the peptide in the gut of foetuses and adults. The observed heterogeneity of Tβ4 expression in the foetal life, ranging from a very rare detection in liver cells up to a diffuse reactivity in endocrine pancreas, should be taken into account when the role of Tβ4 in the development of human embryo is assessed. Future studies are needed to shed light on the link between Tβ4 and organogenesis.
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