Colloidal Gels with Extracellular Matrix Particles and Growth Factors for Bone Regeneration in Critical Size Rat Calvarial Defects.
Colloidal Gels with Extracellular Matrix Particles and Growth Factors for Bone Regeneration in Critical Size Rat Calvarial Defects.
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DOI：
10.1208/s12248-017-0045-0
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发表时间：
2017-05
期刊：
影响因子：
--
通讯作者：
Berkland CJ
中科院分区：
文献类型：
--
作者：
Townsend JM;Dennis SC;Whitlow J;Feng Y;Wang J;Andrews B;Nudo RJ;Detamore MS;Berkland CJ
胶体凝胶封装天然材料，并表现出糊状性质的放置是有前途的填充复杂的几何形状在颅面骨再生应用。胶体材料在骨科应用中作为骨替代物的临床结果适中，但在颅面应用中的成功有限。因此，新型胶体凝胶的开发将填补用于颅面重建的市售产品的空白。这项技术的一个可能的应用是颅骨重建。目前，创伤性脑损伤（TBI）通常采用半颅骨切除术治疗，这是一种切除一半颅骨以使受伤的大脑肿胀并疝出封闭的颅穹窿的手术。胶体凝胶的使用将允许设计能够在脑肿胀期间膨胀的柔韧材料，并促进颅骨再生，从而减轻对第二次手术以替换先前移除的半颅骨的需要。在当前的研究中，羟基磷灰石（HAp），脱矿骨基质（DBM）和脱细胞软骨（DCC）的胶体纳米颗粒与透明质酸（HA）结合，形成具有理想的流变性能（τy ≥ 100 Pa）的胶体凝胶。包括BMP-2和VEGF生长因子以评估DBM和DCC的细胞外基质（ECM）贡献。与假手术组相比，HA-HAp（BMP-2）和HA-HAp-DCC组的骨再生分别高出89%和82%（p < 0.01）。观察到的材料保留问题可以通过实施化学交联来缓解。总的来说，DCC可能是一种有前途的骨再生材料，胶体凝胶可能在颅面应用中具有显着的潜力。
Colloidal gels encapsulating natural materials and exhibiting paste-like properties for placement are promising for filling complex geometries in craniofacial bone regeneration applications. Colloidal materials have demonstrated modest clinical outcomes as bone substitutes in orthopedic applications, but limited success in craniofacial applications. As such, development of a novel colloidal gel will fill a void in commercially available products for use in craniofacial reconstruction. One likely application for this technology is cranial reconstruction. Currently, traumatic brain injury (TBI) is often treated with a hemicraniectomy, a procedure in which half the cranium is removed to allow the injured brain to swell and herniate beyond the enclosed cranial vault. The use of colloidal gels would allow for the design of a pliable material capable of expansion during brain swelling and facilitate cranial bone regeneration alleviating the need for a second surgery to replace the previously removed hemi-cranium. In the current study, colloidal nanoparticles of hydroxyapatite (HAp), demineralized bone matrix (DBM), and decellularized cartilage (DCC) were combined with hyaluronic acid (HA) to form colloidal gels with desirable rheological properties (τy ≥ 100 Pa). BMP-2 and VEGF growth factors were included to assess extracellular matrix (ECM) contribution of DBM and DCC. The HA-HAp (BMP-2) and HA-HAp-DCC group had 89 and 82% higher bone regeneration compared to the sham group, respectively (p < 0.01). Material retention issues observed may be alleviated by implementing chemical crosslinking. Overall, DCC may be a promising material for bone regeneration in general, and colloidal gels may hold significant potential in craniofacial applications.
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影响因子：
4.1
作者：
Patel, Zarana S.;Young, Simon;Tabata, Yasuhiko;Jansen, John A.;Wong, Mark E. K.;Mikos, Antonios G.
通讯作者：
Mikos, Antonios G.
基于生物活性的微球支架，含有脱细胞软骨。
影响因子：
4.6
作者：
Sutherland AJ;Detamore MS
通讯作者：
Detamore MS
影响因子：
14
作者：
Visser, Jetze;Gawlitta, Debby;Malda, Jos
通讯作者：
Malda, Jos
影响因子：
--
作者：
Schiffer, J;Gur, R;Pollak, L
通讯作者：
Pollak, L
DOI：
10.1021/la4041985
发表时间：
2014-04-01
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
--
作者：
Dennis SC;Detamore MS;Kieweg SL;Berkland CJ
通讯作者：
Berkland CJ