Mapping glycosaminoglycan-hydroxyapatite colloidal gels as potential tissue defect fillers.
Mapping glycosaminoglycan-hydroxyapatite colloidal gels as potential tissue defect fillers.
复制标题
DOI：
10.1021/la4041985
复制
发表时间：
2014-04-01
期刊：
影响因子：
--
通讯作者：
Berkland CJ
中科院分区：
文献类型：
--
作者：
Dennis SC;Detamore MS;Kieweg SL;Berkland CJ
可延展的生物材料如Herschel-Bulkley（H-B）流体具有剪切响应流变性质，并且能够在机械破坏后自组装和粘弹性恢复（例如，通过注射或扩散的外科放置）。这项研究表明，加入中等分子量的糖胺聚糖（GAG），如硫酸软骨素（CS），（Mw = 15-30 kDa）和透明质酸（HA）（Mw = 20-41 kDa）可用于改变几种流变性能，包括稠度指数（K），流动行为指数（n），亚微米羟基磷灰石（HAP）（Davg ≤ 200 nm）胶体凝胶的屈服应力（τy）。GAG-HAP胶体混合物表现出大量的聚合物-颗粒协同作用，可能是由于“桥接”絮凝，这导致稠度指数（KGAG-HAP ≥ KGAG + KHAP）的协同增加，而不损害凝胶的剪切稀化行为（n < 1）。此外，含有高浓度HAP（60- 80%w/v）的GAG-HAP胶体表现出显著的屈服应力（τy ≥ 100 Pa）和粘弹性恢复特性（G′恢复≥ 64%）。虽然在CS-HAP和HA-HAP胶体凝胶之间观察到流变学差异，但CS和HA都代表了涉及骨缺损填充的未来研究的可行选择。总的来说，本研究确定了混合物区域，其中CS-HAP和HA-HAP胶体凝胶中的流变学性质与所需性质一致，以便于在非承重组织填充应用中进行手术放置，例如颅骨缺损。
Malleable biomaterials such as Herschel–Bulkley (H–B) fluids possess shear responsive rheological properties and are capable of self-assembly and viscoelastic recovery following mechanical disruption (e.g., surgical placement via injection or spreading). This study demonstrated that the addition of moderate molecular weight glycosaminoglycans (GAGs) such as chondroitin sulfate (CS) (Mw = 15–30 kDa) and hyaluronic acid (HA) (Mw = 20–41 kDa) can be used to modify several rheological properties including consistency index (K), flow-behavior index (n), and yield stress (τy) of submicrometer hydroxyapatite (HAP) (Davg ≤ 200 nm) colloidal gels. GAG–HAP colloidal mixtures exhibited substantial polymer–particle synergism, likely due to “bridging” flocculation, which led to a synergistic increase in consistency index (KGAG-HAP ≥ KGAG + KHAP) without compromising shear-thinning behavior (n < 1) of the gel. In addition, GAG–HAP colloids containing high concentrations of HAP (60–80% w/v) exhibited substantial yield stress (τy ≥ 100 Pa) and viscoelastic recovery properties (G′recovery ≥ 64%). While rheological differences were observed between CS–HAP and HA–HAP colloidal gels, both CS and HA represent feasible options for future studies involving bone defect filling. Overall, this study identified mixture regions where rheological properties in CS–HAP and HA–HAP colloidal gels aligned with desired properties to facilitate surgical placement in non-load-bearing tissue-filling applications such as calvarial defects.
登录
查看更多内容
DOI：
10.1023/a:1015773322821
发表时间：
2002-07-01
影响因子：
3.7
作者：
Bao, Y;Senos, AMR;Gauckler, LJ
通讯作者：
Gauckler, LJ
影响因子：
3.9
作者：
Gardini, D;Galassi, C;Lapasin, R
通讯作者：
Lapasin, R
影响因子：
9.7
作者：
Habib, M.;Baroud, G.;Bohner, M.
通讯作者：
Bohner, M.
一种骨状纳米羟基磷灰石/胶原蛋白负载的可注射支架
影响因子：
4
作者：
Huang, Zhi;Tian, Jing;Feng, Qingling
通讯作者：
Feng, Qingling
影响因子：
14
作者：
Murugan, R;Ramakrishna, S
通讯作者：
Ramakrishna, S