Bienzymatic glucose biosensor based on three dimensional macroporous ionic liquid doped sol-gel organic-inorganic composite
Bienzymatic glucose biosensor based on three dimensional macroporous ionic liquid doped sol-gel organic-inorganic composite
复制标题
基于三维大孔离子液体掺杂溶胶-凝胶有机无机复合材料的双酶葡萄糖生物传感器
DOI：
10.1016/j.snb.2012.01.053
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发表时间：
2012-03
期刊：
影响因子：
--
通讯作者：
Yao, Cheng
中科院分区：
文献类型：
--
作者：
Chen, Xiaojun;Zhu, Jinwei;Tian, Rong;Yao, Cheng
制备了一种新型的三维大孔离子液体(IL)掺杂聚(N[3-三甲氧基硅基)丙基]苯胺(PTMSPA)反蛋白石薄膜。该制备过程采用了自组装的一锅法电化学工艺，避免了多步骤过程的繁琐。PTMSPA在二氧化硅骨架中具有硅酸盐网络和共价连接的聚苯胺(PANI)链。3 DM-PTMSPA-IL修饰电极的性能优于各组分。将辣根过氧化物酶(HRP)和葡萄糖氧化酶(GOD)固定在含有Nafion的电活性膜上，将修饰电极命名为NF-GOD-HRP/3 DM-PTMSPA-IL/ITO。通过电化学阻抗谱(EIS)、循环伏安法(CV)和计时电流法研究了该生物传感器的电化学行为。在优化的条件下，生物电催化响应电流在0.05~1.0 mM和1.0~8.0 mM的葡萄糖浓度范围内呈良好的线性关系，检出限为0.01 mM。此外，对人血清中葡萄糖水平的检测结果与临床方法符合良好。
A novel three dimensional macroporous (3DM) ionic liquid (IL) doped poly (N[3-(trimethoxysilyl)propyl]aniline) (PTMSPA) inverse opal film was presented. The fabrication involved a self-assembly directed one-pot electrochemical process, which avoided the tediousness in the multi-step procedures. PTMSPA has the silicate network as well as the covalently linked polyaniline (PANI) chains within silica framework. The 3DM-PTMSPA-IL modified electrode showed superior performances over the individual components. Bienzymes, horseradish peroxidase (HRP) and glucose oxidase (GOD) were immobilized on the electroactive film with Nafion (Nf), thus, the modified electrode was designated as Nf-GOD-HRP/3DM-PTMSPA-IL/ITO. Electrochemical behavior of the biosensor was investigated by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and chronoamperometry. Under the optimized conditions, the bioelectrocatalytic response currents revealed good linear relationship in the glucose concentration ranges of 0.05–1.0mM and 1.0–8.0mM with a detection limit of 0.01mM. Furthermore, the detection of glucose levels in human sera showed satisfactory agreement with the clinical method.
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影响因子：
6.6
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通讯作者：
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