Transcriptomic changes in the nasal epithelium associated with diesel engine exhaust exposure.
Transcriptomic changes in the nasal epithelium associated with diesel engine exhaust exposure.
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DOI：
10.1016/j.envint.2020.105506
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发表时间：
2020-04
影响因子：
11.8
通讯作者：
Lan Q
中科院分区：
文献类型：
--
作者：
Drizik E;Corbett S;Zheng Y;Vermeulen R;Dai Y;Hu W;Ren D;Duan H;Niu Y;Xu J;Fu W;Meliefste K;Zhou B;Zhang X;Yang J;Bassig B;Liu H;Ye M;Liu G;Jia X;Meng T;Bin P;Zhang J;Silverman D;Spira A;Rothman N;Lenburg ME;Lan Q
Diesel engine exhaust (DEE) exposure causes lung cancer, but the molecular mechanisms by which this occurs are not well understood. To assess transcriptomic alterations in nasal epithelium of DEE-exposed factory workers to better understand the cellular and molecular effects of DEE. Nasal epithelial brushings were obtained from 41 diesel engine factory workers exposed to relatively high levels of DEE (17.2 – 105.4 ug/m3), and 38 unexposed workers from factories without DEE exposure. mRNA was profiled for gene expression using Affymetrix microarrays. Linear modeling was used to identify differentially expressed genes associated with DEE exposure and interaction effects with current smoking status. Pathway enrichment among differentially expressed genes was assessed using EnrichR. Gene Set Enrichment Analysis (GSEA) was used to compare gene expression patterns between datasets. 225 genes had expression associated with DEE exposure after adjusting for smoking status (FDR q < 0.25) and were enriched for genes in pathways related to oxidative stress response, cell cycle pathways such as MAPK/ERK, protein modification, and transmembrane transport. Genes up-regulated in DEE-exposed individuals were enriched among the genes most up-regulated by cigarette smoking in a previously reported bronchial airway smoking dataset. We also found that the DEE signature was enriched among the genes most altered in two previous studies of the effects of acute DEE on PBMC gene expression. An exposure-response relationship was demonstrated between air levels of elemental carbon and the first principal component of the DEE signature. A gene expression signature was identified for workers occupationally exposed to DEE that was altered in an exposure-dependent manner and had some overlap with the effects of smoking and the effects of acute DEE exposure. This is the first study of gene expression in nasal epithelial cells of workers heavily exposed to DEE and provides new insights into the molecular alterations that occur with DEE exposure.
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影响因子：
14.9
作者：
Kuleshov MV;Jones MR;Rouillard AD;Fernandez NF;Duan Q;Wang Z;Koplev S;Jenkins SL;Jagodnik KM;Lachmann A;McDermott MG;Monteiro CD;Gundersen GW;Ma'ayan A
通讯作者：
Ma'ayan A
DOI：
10.1007/s11356-017-9995-0
发表时间：
2017-11
期刊：
Environmental science and pollution research international
影响因子：
--
作者：
Kowalska M;Wegierek-Ciuk A;Brzoska K;Wojewodzka M;Meczynska-Wielgosz S;Gromadzka-Ostrowska J;Mruk R;Øvrevik J;Kruszewski M;Lankoff A
通讯作者：
Lankoff A
影响因子：
3.8
作者：
Koike, E;Hirano, S;Kobayashi, T
通讯作者：
Kobayashi, T
影响因子：
2.8
作者：
Dai Y;Ren D;Bassig BA;Vermeulen R;Hu W;Niu Y;Duan H;Ye M;Meng T;Xu J;Bin P;Shen M;Yang J;Fu W;Meliefste K;Silverman D;Rothman N;Lan Q;Zheng Y
通讯作者：
Zheng Y
影响因子：
2.5
作者：
Lechasseur A;Jubinville É;Routhier J;Bérubé JC;Hamel-Auger M;Talbot M;Lamothe J;Aubin S;Paré MÈ;Beaulieu MJ;Bossé Y;Duchaine C;Morissette MC
通讯作者：
Morissette MC