Aluminum ions stimulate mitosis in murine cells in tissue culture
Aluminum ions stimulate mitosis in murine cells in tissue culture
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铝离子刺激组织培养中小鼠细胞的有丝分裂
DOI：
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发表时间：
1986
影响因子：
4
通讯作者：
T. Reid
中科院分区：
文献类型：
--
作者：
T. Jones;Donna L. Antonetti;T. Reid
向中野小鼠透镜上皮（NMLE）细胞和Swiss 3 T3 K细胞的培养基中添加铝可诱导3 H-胸苷掺入和有丝分裂。这与其他金属离子如钒形成对比，钒在足以增加3 H-胸苷掺入的浓度下实际上抑制有丝分裂（Jones和Reid，J Cell Physiol 121：199，1984 [1]）。铝浓度在20 μM和50 μM之间最有效。3 T3细胞对铝的反应增加了7.6倍，NMLE细胞对3 H-胸苷掺入的反应增加了21倍。NMLE细胞中的DNA合成也被铝和低浓度胰岛素（4.5 × 10−8 M）协同刺激。用50 μM铝孵育3.25 h足以诱导40 h试验期间最大3 H-胸苷掺入量的50%。铝刺激的3 H-胸苷掺入被羟基脲抑制，铝导致细胞数量增加。此外，通过对铝刺激的DNA合成产物的沉降平衡分析，发现在向静止细胞中添加铝后合成了单拷贝的DNA。这些事实表明，铝诱导S期DNA合成和有丝分裂。然而，只有48%的被DNA标记的NMLE细胞继续分裂。相比之下，虽然只有一小部分的3 T3细胞被发现铝处理后被标记，所有这些细胞似乎通过有丝分裂。
Addition of aluminum to the culture medium of Nakano mouse lens epithelial (NMLE) cells and Swiss 3T3K cells induced both 3H‐thymidine incorporation and mitosis. This is in contrast to other metal ions such as vanadium, which, at concentrations high enough to increase 3H‐thymidine incorporation, actually inhibits mitosis (Jones and Reid, J Cell Physiol 121:199, 1984 [1]). Aluminum concentrations between 20 μM and 50 μM were most effective. The 3T3 cells respond to aluminum with a 7.6‐fold increase, and NMLE cells respond with a 21‐fold increase in 3H‐thymidine incorporation. DNA synthesis in NMLE cells was also found to be synergistically stimulated by aluminum and low concentrations of insulin (4.5 × 10−8 M). A 3.25‐hr incubation with 50 μM aluminum was sufficient to induce 50% of maximum 3H‐thymidine incorporation during the 40‐hr assay. Aluminum‐stimulated 3H‐thymidine incorporation is inhibited by hydroxyurea, and aluminum causes an increase in cell number. Also, by sedimentation equilibrium analysis of the product of aluminum‐stimulated DNA synthesis it was found that a single copy of DNA was synthesized following addition of aluminum to quiescent cells. These facts indicate that aluminum induces both S‐phase DNA synthesis and mitosis. However, only 48% of the NMLE cells found to be labeled with DNA went on to divide. In contrast, although only a small percentage of 3T3 cells were found to be labeled after aluminum treatment, all of these cells appeared to go through mitosis.
影响因子：
56.9
作者：
PERL, DP;GAJDUSEK, DC;GIBBS, CJ
通讯作者：
GIBBS, CJ
DOI：
10.1073/pnas.79.16.4888
发表时间：
1982-08
影响因子：
11.1
作者：
P. Sternweis;A. Gilman
通讯作者：
P. Sternweis;A. Gilman