Optimized superficially porous particles for protein separations.
Optimized superficially porous particles for protein separations.
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DOI：
10.1016/j.chroma.2013.09.054
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发表时间：
2013-11-08
期刊：
影响因子：
--
通讯作者：
Kirkland JJ
中科院分区：
文献类型：
--
作者：
Schuster SA;Wagner BM;Boyes BE;Kirkland JJ
制药和生物技术公司对更大的治疗分子的持续兴趣提供了对用于在发现阶段和随后的质量控制期间检查这些分子的改进工具的需要。为了满足这一需求，已经开发了具有适当表面性质的较大孔表面多孔颗粒（Fused-Core®颗粒），其孔径为400 μ m，允许大分子（<500 kDa）不受限制地进入键合相。此外，采用的粒度（3.4 μm）可实现适用于潜在压力敏感蛋白质的高效低压分离。对新的熔核颗粒的壳厚度的研究表明，在短扩散路径和高效率与足够的保持力和质量负荷容限之间进行了折衷。此外，蛋白质的反相分离的上级性能要求结合相的特定设计特性。结果，具有独特键合相的新颗粒的柱显示出优异的稳定性和与质谱法适用的移动的相的高相容性。该报告包括完整蛋白质混合物的快速分离，以及较大单克隆抗体材料和相关变体的极高分辨率分离的示例。显示了蛋白质回收率、样品加载量和分析动态范围的调查。证明了这些新的400 μ m熔融核心颗粒，专门设计用于蛋白质分析，超过传统的蛋白质分离颗粒的优势。
Continuing interest in larger therapeutic molecules by pharmaceutical and biotech companies provides the need for improved tools for examining these molecules both during the discovery phase and later during quality control. To meet this need, larger pore superficially porous particles with appropriate surface properties (Fused-Core® particles) have been developed with a pore size of 400 Å, allowing large molecules (<500 kDa) unrestricted access to the bonded phase. In addition, a particle size (3.4 μm) is employed that allows high-efficiency, low-pressure separations suitable for potentially pressure-sensitive proteins. A study of the shell thickness of the new fused-core particles suggests a compromise between a short diffusion path and high efficiency versus adequate retention and mass load tolerance. In addition, superior performance for the reversed-phase separation of proteins requires that specific design properties for the bonded-phase should be incorporated. As a result, columns of the new particles with unique bonded phases show excellent stability and high compatibility with mass spectrometry-suitable mobile phases. This report includes fast separations of intact protein mixtures, as well as examples of very high-resolution separations of larger monoclonal antibody materials and associated variants. Investigations of protein recovery, sample loading and dynamic range for analysis are shown. The advantages of these new 400 Å fused-core particles, specifically designed for protein analysis, over traditional particles for protein separations are demonstrated.
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影响因子：
3.1
作者：
Baker, Jared S.;Vinci, John C.;Colon, Luis A.
通讯作者：
Colon, Luis A.
影响因子：
7.4
作者：
KIRKLAND, JJ
通讯作者：
KIRKLAND, JJ
影响因子：
7.4
作者：
KIRKLAND, JJ;GLAJCH, JL;FARLEE, RD
通讯作者：
FARLEE, RD
DOI：
10.1016/j.jpba.2009.05.011
发表时间：
2009-10-15
影响因子：
3.4
作者：
Song, Wei;Pabbisetty, Deepthi;Fast, Douglas M.
通讯作者：
Fast, Douglas M.
影响因子：
1.3
作者：
KIRKLAND, JJ
通讯作者：
KIRKLAND, JJ