Robust Cell Detection for Large-Scale 3D Microscopy Using GPU-Accelerated Iterative Voting.

Robust Cell Detection for Large-Scale 3D Microscopy Using GPU-Accelerated Iterative Voting.
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DOI:
10.3389/fnana.2018.00028
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发表时间:
2018
影响因子:
2.9
通讯作者:
Mayerich D
Mayerich D
中科院分区:
医学3区
文献类型:
--
作者:
Saadatifard L;Abbott LC;Montier L;Ziburkus J;Mayerich D

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High-throughput imaging techniques, such as Knife-Edge Scanning Microscopy (KESM),are capable of acquiring three-dimensional whole-organ images at sub-micrometer resolution. These images are challenging to segment since they can exceed several terabytes (TB) in size, requiring extremely fast and fully automated algorithms. Staining techniques are limited to contrast agents that can be applied to large samples and imaged in a single pass. This requires maximizing the number of structures labeled in a single channel, resulting in images that are densely packed with spatial features. In this paper, we propose a three-dimensional approach for locating cells based on iterative voting. Due to the computational complexity of this algorithm, a highly efficient GPU implementation is required to make it practical on large data sets. The proposed algorithm has a limited number of input parameters and is highly parallel.
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