Action mechanisms of lithium chloride on cell infection by transmissible gastroenteritis coronavirus.
Action mechanisms of lithium chloride on cell infection by transmissible gastroenteritis coronavirus.
复制标题
氯化锂对传染性胃肠炎冠状病毒细胞感染的作用机制
DOI：
10.1371/journal.pone.0018669
复制
发表时间：
2011-05-06
期刊：
影响因子：
3.7
通讯作者：
Herrler G
中科院分区：
文献类型：
--
作者：
Ren X;Meng F;Yin J;Li G;Li X;Wang C;Herrler G
猪传染性胃肠炎病毒（TGEV）是一种猪冠状病毒。氯化锂（LiCl）已被发现对几种DNA病毒有效，如单纯疱疹病毒和牛痘病毒。最近，我们和其他人报道了氯化锂对鸡传染性支气管炎冠状病毒（IBV）感染的抑制作用。本研究探讨了氯化锂对TGEV感染细胞的作用机制。空斑试验和3-（4，5）-二甲基噻唑（-z-y 1）-3，5-二苯基四氮唑溴化物（MTT）试验表明，当将LiCl加入病毒感染的细胞中时，TGEV对细胞的感染以剂量依赖的方式受到抑制;当细胞或病毒用药物预处理时，细胞感染不受影响。观察了氯化锂在不同病毒剂量和不同细胞系下对TGEV体外感染的抑制作用。通过RT-PCR和实时荧光定量PCR检测TGEV S和3CL蛋白酶基因，证实了LiCl对TGEV感染和转录的抑制作用。药物对TGEV感染的时间效应表明，LiCl作用于TGEV感染的初期和晚期。由于药物处理，在感染后36 h未检测到病毒产生。此外，免疫荧光（IF）和流式细胞仪分析的基础上的Annexin V染色和碘化丙啶染色的细胞核表明，TGEV诱导的早期和晚期细胞凋亡被有效地抑制。通过caspase-3表达的IF分析研究LiCl抑制细胞凋亡的能力。我们的数据表明，氯化锂抑制TGEV感染，发挥抗凋亡作用。同时用猪流行性腹泻冠状病毒观察了氯化锂的抑制作用。结合其他关于锂盐在细胞培养中对IBV的抑制作用的报道，我们的研究结果表明，LiCl可能是一种有效的抗猪和禽冠状病毒的药物。
Transmissible gastroenteritis virus (TGEV) is a porcine coronavirus. Lithium chloride (LiCl) has been found to be effective against several DNA viruses, such as Herpes simplex virus and vaccinia virus. Recently, we and others have reported the inhibitory effect of LiCl on avian infectious bronchitis coronavirus (IBV) infection, an RNA virus. In the current study, the action mechanism of LiCl on cell infection by TGEV was investigated. Plaque assays and 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenyl tetrazoliumbromide (MTT) assays showed that the cell infection by TGEV was inhibited in a dose-dependent manner, when LiCl was added to virus-infected cells; the cell infection was not affected when either cells or viruses were pretreated with the drug. The inhibition of TGEV infection in vitro by LiCl was observed at different virus doses and with different cell lines. The inhibitory effect of LiCl against TGEV infection and transcription was confirmed by RT-PCR and real-time PCR targeting viral S and 3CL-protease genes. The time-of-addition effect of the drug on TGEV infection indicated that LiCl acted on the initial and late stage of TGEV infection. The production of virus was not detected at 36 h post-infection due to the drug treatment. Moreover, immunofluorescence (IF) and flow cytometry analyses based on staining of Annexin V and propidium iodide staining of nuclei indicated that early and late cell apoptosis induced by TGEV was inhibited efficiently. The ability of LiCl to inhibit apoptosis was investigated by IF analysis of caspase-3 expression. Our data indicate that LiCl inhibits TGEV infection by exerting an anti-apoptotic effect. The inhibitory effect of LiCl was also observed with porcine epidemic diarrhea coronavirus. Together with other reports concerning the inhibitory effect of lithium salts on IBV in cell culture, our results indicate that LiCl may be a potent agent against porcine and avian coronaviruses.
登录
查看更多内容
DOI：
10.1016/j.bbrc.2004.05.107
发表时间：
2004-07-09
影响因子：
3.1
作者：
Mizutani T;Fukushi S;Saijo M;Kurane I;Morikawa S
通讯作者：
Morikawa S
影响因子：
5.4
作者：
Liu, C;Xu, HY;Liu, DX
通讯作者：
Liu, DX
影响因子：
5.4
作者：
Schwegmann-Wessels, C;Zimmer, G;Herrler, G
通讯作者：
Herrler, G
影响因子：
2.7
作者：
Musial, BL;Chojnacki, JK;Coleman, CI
通讯作者：
Coleman, CI
胆固醇对于传染性胃肠炎病毒感染细胞的重要性
影响因子：
5
作者：
Ren X;Glende J;Yin J;Schwegmann-Wessels C;Herrler G
通讯作者：
Herrler G