Effects of pre-eclampsia on HDL-mediated cholesterol efflux capacity after pregnancy.
Effects of pre-eclampsia on HDL-mediated cholesterol efflux capacity after pregnancy.
复制标题
DOI：
10.1016/j.athplu.2022.01.003
复制
发表时间：
2022-04
期刊：
影响因子：
--
通讯作者：
Kritharides, Leonard
中科院分区：
文献类型：
--
作者：
Kockx, Maaike;Roberts, Lynne;Wang, Jeffrey;Tran, Collin;Brown, Mark A.;Kritharides, Leonard
子痫前期(PE)与心血管疾病的风险终身增加有关。高密度脂蛋白(高密度脂蛋白)的主要保护功能之一是它在胆固醇反向运输中的作用。妊娠期PE患者高密度脂蛋白介导的胆固醇流出能力(CEC)降低。这种情况在产后是否会持续还不得而知。对血压正常的孕妇(n=44)和PE孕妇(n=42)产后6个月测定基础CEC和转运体特异性CEC。23名血压正常的妇女和20名正常的PE妇女在产后24个月采集样本，也测量了CEC。用稳定表达人ABCA1或Abcg1的中国仓鼠卵巢细胞初步测定了基础的、ATP结合盒转运体A1(ABCA1)和G1(Abcg1)特异的CEC，并用J774小鼠巨噬细胞株进行了鉴定。在产后6个月进行PE的妇女中，ABCA1特异性CEC显著降低(0.57±0.1vs0.53±0.08；p<0.05)，而基础和Abcg1特异性外排无显著差异。6个月后，J774细胞内cAMP特异性CEC也降低(0.85±0.21 vs 0.75±0.0.25，p<0.05)。尽管与对照组相比，PE患者的载脂蛋白A-I、载脂蛋白E、纤溶酶原和PON-1水平无显著差异，但ABCA1流出与正常后的载脂蛋白A-L、高密度脂蛋白胆固醇和载脂蛋白E水平以及妊娠后的载脂蛋白A-L和高密度脂蛋白胆固醇水平呈正相关。在所有产后6至24个月的女性中，ABCA1特异性外排减少了11%±11.6%，在正常血压妊娠的女性中下降了9%±71.3%，在进行PE的女性中下降了9%±11.3%。在调整apoA-I水平后，产后6个月组和血压正常的妇女组之间ABCA1特异性外排随时间的推移没有显著差异，但在进行PE的妇女中，6个月和24个月之间仍然存在显著差异。ABCA1介导的CEC在PE妊娠后6个月受损，之后在正常血压和PE妊娠中均下降。ABCA1介导的外排在怀孕后是动态的，但不太可能解释患有PE的女性心血管疾病风险的长期增加。有先兆子痫病史的女性表现出高密度脂蛋白介导的胆固醇外流减少。先兆子痫后胆固醇外流受损与ABCA1转运蛋白有关。高密度脂蛋白的功能在怀孕后是动态的。先兆子痫后胆固醇外流受损并不能解释一生中心血管疾病风险的增加。
Preeclampsia (PE) is associated with life-long increased risk of cardiovascular disease. One of the main protective functions of high-density lipoprotein (HDL) is its role in reverse cholesterol transport. HDL-mediated cholesterol efflux capacity (CEC) is decreased during pregnancy in women with PE. Whether this persists postpartum is unknown. Basal and transporter-specific CEC were determined 6 months postpartum in women who had a normotensive (n = 44) or a PE (n = 42) pregnancy. CEC was also measured in 23 normotensive and 20 PE women for whom samples were collected 24 months postpartum. Basal, ATP-binding cassette transporter-A1 (ABCA1)- and -G1 (ABCG1)-specific CEC were primarily determined using Chinese hamster ovary cells stably expressing human ABCA1 or ABCG1, and were also assessed using a J774 mouse macrophage cell line. ABCA1-specific CEC was significantly lower in women who had PE 6 months postpartum (0.57 ± 0.1 vs 0.53 ± 0.08; p < 0.05), whilst basal and ABCG1-specific efflux were not significantly different. cAMP-specific CEC in J774 cells was also lower 6 months after PE (0.85 ± 0.21 vs 0.75 ± 0.25, p < 0.05). Although apoA-I, apoE, plasminogen and PON-1 levels were not significantly different in women who had PE compared with controls, ABCA1 efflux did correlate with apoA-l, HDL-C and apoE levels after a normal, and with apoA-l and HDL-C levels after a PE pregnancy. ABCA1-specific efflux decreased in all women between 6 and 24 months postpartum, by 11 ± 1.6% in women who had a normotensive pregnancy and 9 ± 1.3% in women who had PE. After adjustment for apoA-I levels, there was no significant difference in ABCA1-specific efflux between the groups at 6 months postpartum and in normotensive women over time, but remained significantly different between 6 and 24 months in women who had PE. ABCA1-mediated CEC is impaired 6 months postpartum after a PE pregnancy and decreases thereafter in both normotensive and PE pregnancies. ABCA1-mediated efflux is dynamic after pregnancy but is unlikely to explain the long-term increased CVD risk in women with PE. Women with a history of preeclampsia display decreased HDL-mediated cholesterol efflux. Impaired cholesterol efflux after preeclampsia is specific to the ABCA1-transporter. HDL function is dynamic after pregnancy. Impaired cholesterol efflux after preeclampsia does not explain life-long increased risk of CVD.
登录
查看更多内容
影响因子：
2.8
作者：
IGLESIAS, A;MONTELONGO, A;LASUNCION, MA
通讯作者：
LASUNCION, MA
DOI：
10.1161/atvbaha.120.314847
发表时间：
2020-11-01
影响因子：
8.7
作者：
Luquain-Costaz, Celine;Kockx, Maaike;Kritharides, Leonard
通讯作者：
Kritharides, Leonard
影响因子：
2.6
作者：
Einbinder，Yael;Biron-Shental，Tal;Zitman-Gal，Tali
通讯作者：
Zitman-Gal，Tali
影响因子：
5.3
作者：
Akhter, Tansim;Larsson, Anders;Naessen, Tord
通讯作者：
Naessen, Tord
DOI：
10.1161/atvbaha.109.199158
发表时间：
2010-04
期刊：
Arteriosclerosis, thrombosis, and vascular biology
影响因子：
--
作者：
de la Llera-Moya M;Drazul-Schrader D;Asztalos BF;Cuchel M;Rader DJ;Rothblat GH
通讯作者：
Rothblat GH