SlARF2a plays a negative role in mediating axillary shoot formation.
SlARF2a plays a negative role in mediating axillary shoot formation.
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SlARF2a 在介导腋芽形成中发挥负面作用
DOI：
10.1038/srep33728
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发表时间：
2016-09-20
影响因子：
4.6
通讯作者：
Li T
中科院分区：
文献类型：
--
作者：
Xu T;Liu X;Wang R;Dong X;Guan X;Wang Y;Jiang Y;Shi Z;Qi M;Li T
SLARF2a在大多数植物器官中都有表达，包括根、叶、花和果实。详细的表达研究表明，SlARF2a主要在叶节和节间横截面中表达，表明SLARF2a的表达仅限于维管器官。断头或应用6-苄基氨基嘌呤(6-BAP)最初可促进腋芽的生长，在此期间SLARF2a的表达显著降低。下调SLARF2a的表达导致双子叶植物的频率增加，并显著促进侧器官的发育。茎解剖研究表明，番茄植株的形成层和韧皮部表达下调的ARF2a水平，这与生长素分布的明显变化有关。进一步的分析表明，生长素运输的改变可能是通过改变PIN的表达来实现的。为了确定AUX/IAA和TPL与ARF2a的相互作用，研究了在腋芽发育过程中下调的4个腋芽发育抑制因子IAA3、IAA9、SLTPL1和SLTPL6与ARF2a的直接相互作用。虽然这些抑制子都不直接参与ARF2a的活性，但IAA3、IAA9和ARF2a的相似表达模式表明它们可能在腋芽形成和其他发育过程中密切相关。
SlARF2a is expressed in most plant organs, including roots, leaves, flowers and fruits. A detailed expression study revealed that SlARF2a is mainly expressed in the leaf nodes and cross-sections of the nodes indicated that SlARF2a expression is restricted to vascular organs. Decapitation or the application of 6-benzylaminopurine (BAP) can initially promote axillary shoots, during which SlARF2a expression is significantly reduced. Down-regulation of SlARF2a expression results in an increased frequency of dicotyledons and significantly increased lateral organ development. Stem anatomy studies have revealed significantly altered cambia and phloem in tomato plants expressing down-regulated levels of ARF2a, which is associated with obvious alterations in auxin distribution. Further analysis has revealed that altered auxin transport may occur via altered pin expression. To identify the interactions of AUX/IAA and TPL with ARF2a, four axillary shoot development repressors that are down-regulated during axillary shoot development, IAA3, IAA9, SlTPL1 and SlTPL6, were tested for their direct interactions with ARF2a. Although none of these repressors are directly involved in ARF2a activity, similar expression patterns of IAA3, IAA9 and ARF2a implied they might work tightly in axillary shoot formation and other developmental processes.
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影响因子：
6.9
作者：
Hao Y;Wang X;Li X;Bassa C;Mila I;Audran C;Maza E;Li Z;Bouzayen M;van der Rest B;Zouine M
通讯作者：
Zouine M
影响因子：
4.5
作者：
Breitel DA;Chappell-Maor L;Meir S;Panizel I;Puig CP;Hao Y;Yifhar T;Yasuor H;Zouine M;Bouzayen M;Granell Richart A;Rogachev I;Aharoni A
通讯作者：
Aharoni A
影响因子：
6.9
作者：
Chaabouni S;Jones B;Delalande C;Wang H;Li Z;Mila I;Frasse P;Latché A;Pech JC;Bouzayen M
通讯作者：
Bouzayen M
影响因子：
2.9
作者：
Deng, Wei;Yan, Fang;Li, Zhengguo
通讯作者：
Li, Zhengguo
影响因子：
4.6
作者：
Berger, Yael;Harpaz-Saad, Smadar;Ori, Naomi
通讯作者：
Ori, Naomi